| Determination of uric acid by an anion-exchange column chromatography. | |
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MedLine Citation:
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PMID: 3619024 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A column chromatography using a conventional anion-exchange resin for the separation of uric acid from other purine metabolites is described. It uses a HCl gradient, and the amount of uric acid is quantified directly by monitoring the absorbance of the effluent at 285 nm. The linear range of response is 0.5 to 100 nmol. The method was applied to the analysis of uric acid in urine and serum. Urine was injected directly into the system, while serum required removal of an interfering substance which absorbs the light and coelutes with uric acid. However, this substance was simply removed by heat coagulation of serum by heating in a boiling water bath for 2 min. |
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Authors:
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D S Hsu; S S Chen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical biochemistry Volume: 163 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1987 May |
Date Detail:
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Created Date: 1987-08-31 Completed Date: 1987-08-31 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 238-41 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anion Exchange Resins Chromatography, Ion Exchange Hot Temperature Humans Urate Oxidase Uric Acid / analysis*, blood, urine |
| Chemical | |
Reg. No./Substance:
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0/Anion Exchange Resins; 69-93-2/Uric Acid; EC 1.7.3.3/Urate Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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