Document Detail


Determination of the underivatized antineoplastic drugs cyclophosphamide and 5-fluorouracil and some of their metabolites by capillary gas chromatography combined with electron-capture and nitrogen-phosphorus selective detection.
MedLine Citation:
PMID:  6672038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid and sensitive method for the determination of cyclophosphamide (CP) and 5-fluorouracil (5-FU) and some of their metabolites in one analysis has been developed. Surface-coated open-tubular OV-275 columns were combined with electron-capture detection and nitrogen-phosphorus selective detection. The influence of the column diameter on the separation is shown. Extraction with 2-propanol-diethyl ether (22:77) allows the isolation of CP, 5-FU and their analogues in one extraction step. The assay was applied to some pharmacokinetic experiments with chemotherapeutically treated patients and with a WAG/Rij rat.
Authors:
E A de Bruin; U R Tjaden; A T van Oosterom; P Leeflang; P A Leclercq
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  279     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1984-05-24     Completed Date:  1984-05-24     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  603-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Gas / methods
Cyclophosphamide / blood*
Electrons
Fluorouracil / blood*
Humans
Nitrogen / analysis
Phosphorus / analysis
Rats
Temperature
Chemical
Reg. No./Substance:
27YLU75U4W/Phosphorus; 8N3DW7272P/Cyclophosphamide; N762921K75/Nitrogen; U3P01618RT/Fluorouracil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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