Document Detail


Determination of underivatized amino acid delta(13)C by liquid chromatography/isotope ratio mass spectrometry for nutritional studies: the effect of dietary non-essential amino acid profile on the isotopic signature of individual amino acids in fish.
MedLine Citation:
PMID:  18473333     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study provides data for the effect of dietary non-essential amino acid composition on the delta(13)C values of individual amino acids in rainbow trout (Oncorhynchus mykiss) using liquid chromatography coupled to isotope ratio mass spectrometry (LC/IRMS). In this experiment, trout were reared either on a control diet or on three experimental diets, differing in the composition of non-essential/conditionally essential amino acids, for a period of 6 weeks. The control diet was a commercial trout starter feed with fish meal as the main protein source. The experimental diets contained no protein, only synthetic amino acids. Diet 1 resembled the composition of fish meal in both essential and non-essential amino acids, Diet 2 had all essential amino acids, but cysteine, glycine, proline and tyrosine were replaced by the corresponding amounts of their precursors, and in Diet 3 all non-essential amino acids were replaced by glutamate. LC/IRMS was used for the determination of delta(13)C values of individual amino acids from diets and tissues without derivatization. Diet affected the delta(13)C of individual amino acids in fish. For fish on Diets 1-3 amino acid delta(13)C values showed a similar trend: phenylalanine showed very little change from diet to body tissue. Arginine, lysine, tyrosine and proline showed strong depletion from diet to body tissue and glycine, alanine, aspartate and serine all showed variable but strong enrichment in (13)C. Improvements are necessary before all amino acid delta(13)C values can be determined; however, this study demonstrates that measuring amino acid isotopic signatures by LC/IRMS is a promising new technique for nutritional physiologists.
Authors:
James McCullagh; Julia Gaye-Siessegger; Ulfert Focken
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  22     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-29     Completed Date:  2008-07-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  1817-22     Citation Subset:  IM    
Affiliation:
Chemistry Research Laboratory, Oxford University, Mansfield Road, Oxford OX1 3TA, UK.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*,  chemistry
Animal Nutritional Physiological Phenomena*
Animals
Carbon Isotopes / analysis
Chromatography, Liquid / methods*
Dietary Proteins / analysis*
Mass Spectrometry / methods*
Time Factors
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbon Isotopes; 0/Dietary Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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