Document Detail


Determination of the ultrastructural pathology of human sperm by atomic force microscopy.
MedLine Citation:
PMID:  11334909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate ultrastructural changes, particularly at the surface, that occur in pathological spermatozoa by using atomic force microscopy (AFM) and to examine the morphological alterations responsible for infertile sperm. DESIGN: Normal fertile and pathological spermatozoa were examined by using a conventional AFM in a noncontact mode. SETTING: Andrology clinic at Los Andes University Hospital. PATIENT(S): Sperm of patients with oligoasthenoteratozoospermia (OAT) and asthenozoospermia were obtained from the Los Andes University Hospital, Mérida, Venezuela. INTERVENTION(S): MAIN OUTCOME MEASURE(S): Morphological details, topological information, and three-dimensional images of the head, neck, and flagellum are presented for both normal and pathological sperm. RESULT(S): The obtained images clearly show dramatic alterations in the morphology of the head, neck, and flagellum of pathological sperm. Even the ultrastructure at the top of the flagellum and the region of the acrosome cap are clearly distinguishable. CONCLUSION(S): This study has significant importance not only for identifying spermatozoa alterations but also for understanding morphological defects and their effects on infertility. If properly exploited, this technique could be an important research tool.
Authors:
N Joshi; H Medina; I Crúz; J Osuna
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Fertility and sterility     Volume:  75     ISSN:  0015-0282     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-03     Completed Date:  2001-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  961-5     Citation Subset:  IM    
Affiliation:
Department of Physiology, Faculty of Medicine, and Laboratory of Andrology, Los Andes University Hospital, Mérida, Venezuela. joshi@ciens.ula.ve
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MeSH Terms
Descriptor/Qualifier:
Humans
Infertility, Male / etiology*,  pathology
Male
Microscopy, Atomic Force
Sperm Tail / pathology,  ultrastructure
Spermatozoa / pathology,  ultrastructure*

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