| Determination of tryptophan, tyrosine, and phenylalanine by second derivative spectrophotometry. | |
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MedLine Citation:
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PMID: 2310197 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Second derivative spectrophotometry has been useful for the determination of aromatic amino acids. However, published methods produce erroneous results, because those methods measure second derivative values by the vertical distance between peak and trough which is subject to variation according to the aromatic amino acid composition of proteins. This paper presents a method of second derivative spectrophotometry which measures second derivative absorbance values by means of the vertical distance from baseline to the derivative curve at a wavelength specifically assigned to each aromatic amino acid, and makes corrections for the interference from other amino acids at the same wavelength. The Appendix describes a computational method for obtaining absolute values of second derivative absorbances directly from normal absorbance values without using the spectrophotometer's derivative mode, because most commercial instruments produce completely arbitrary second derivative values which make comparison of data obtained on two different instruments impossible. |
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Authors:
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Y Nozaki |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 277 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1990 Mar |
Date Detail:
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Created Date: 1990-04-06 Completed Date: 1990-04-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 324-33 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Humans Indicators and Reagents Phenylalanine / analysis* Serum Albumin Serum Albumin, Bovine Spectrophotometry, Ultraviolet / methods Tryptophan / analysis* Tyrosine / analysis* |
| Grant Support | |
ID/Acronym/Agency:
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GM30900/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Serum Albumin; 0/Serum Albumin, Bovine; 55520-40-6/Tyrosine; 63-91-2/Phenylalanine; 73-22-3/Tryptophan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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