Document Detail

Determination of tryptophan, tyrosine, and phenylalanine by second derivative spectrophotometry.
MedLine Citation:
PMID:  2310197     Owner:  NLM     Status:  MEDLINE    
Second derivative spectrophotometry has been useful for the determination of aromatic amino acids. However, published methods produce erroneous results, because those methods measure second derivative values by the vertical distance between peak and trough which is subject to variation according to the aromatic amino acid composition of proteins. This paper presents a method of second derivative spectrophotometry which measures second derivative absorbance values by means of the vertical distance from baseline to the derivative curve at a wavelength specifically assigned to each aromatic amino acid, and makes corrections for the interference from other amino acids at the same wavelength. The Appendix describes a computational method for obtaining absolute values of second derivative absorbances directly from normal absorbance values without using the spectrophotometer's derivative mode, because most commercial instruments produce completely arbitrary second derivative values which make comparison of data obtained on two different instruments impossible.
Y Nozaki
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  277     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-04-06     Completed Date:  1990-04-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  324-33     Citation Subset:  IM    
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
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MeSH Terms
Indicators and Reagents
Phenylalanine / analysis*
Serum Albumin
Serum Albumin, Bovine
Spectrophotometry, Ultraviolet / methods
Tryptophan / analysis*
Tyrosine / analysis*
Grant Support
Reg. No./Substance:
0/Indicators and Reagents; 0/Serum Albumin; 0/Serum Albumin, Bovine; 55520-40-6/Tyrosine; 63-91-2/Phenylalanine; 73-22-3/Tryptophan

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