Document Detail

Determination of trichloroethylene metabolites in rat liver homogenate using headspace gas chromatography.
MedLine Citation:
PMID:  3417823     Owner:  NLM     Status:  MEDLINE    
An headspace gas chromatographic (HSGC) method for determination of trichloroethylene metabolites in rat liver homogenates is described. These metabolites are chloral hydrate (CH), trichloroethanol (TCE), trichloroacetic acid (TCA) and the glucuronic acid conjugate of trichloroethanol (TCE-beta-glucuronide). The method is based on selective thermal conversion of CH and TCA into chloroform, which is determined together with trichloroethanol by HSGC using electron-capture detection. TCE-beta-glucuronide was determined as the difference between free TCE and total TCE after enzymatic hydrolysis with beta-glucuronidase. Synthesized TCE-beta-glucuronide was used to compare the efficiency of enzymatic and acid hydrolysis of the conjugate. Enzymatic hydrolysis was found to be advantageous for determination of TCE-beta-glucuronide.
B Køppen; L Dalgaard; J M Christensen
Related Documents :
16277423 - Thermal oxidation of 9'-cis-neoxanthin in a model system containing peroxyacetic acid l...
6744053 - Different effects of d-glucose anomers on enhanced secretion of gastric acid in rat.
16728583 - Folic acid and its metabolites modulate igf-i receptor gene expression in colon cancer ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  442     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-10-27     Completed Date:  1988-10-27     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  325-32     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chloral Hydrate / analysis
Chromatography, Gas
Hydrogen-Ion Concentration
Liver / analysis*,  metabolism
Rats, Inbred Strains
Trichloroethylene / analysis*
Reg. No./Substance:
290YE8AR51/Trichloroethylene; 418M5916WG/Chloral Hydrate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Automatic headspace gas chromatographic method for the simultaneous determination of trichloroethyle...
Next Document:  High-performance liquid chromatographic determination of aryl N-methylcarbamate residues using post-...