Document Detail


Determination of trichloroethylene metabolites in rat liver homogenate using headspace gas chromatography.
MedLine Citation:
PMID:  3417823     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An headspace gas chromatographic (HSGC) method for determination of trichloroethylene metabolites in rat liver homogenates is described. These metabolites are chloral hydrate (CH), trichloroethanol (TCE), trichloroacetic acid (TCA) and the glucuronic acid conjugate of trichloroethanol (TCE-beta-glucuronide). The method is based on selective thermal conversion of CH and TCA into chloroform, which is determined together with trichloroethanol by HSGC using electron-capture detection. TCE-beta-glucuronide was determined as the difference between free TCE and total TCE after enzymatic hydrolysis with beta-glucuronidase. Synthesized TCE-beta-glucuronide was used to compare the efficiency of enzymatic and acid hydrolysis of the conjugate. Enzymatic hydrolysis was found to be advantageous for determination of TCE-beta-glucuronide.
Authors:
B Køppen; L Dalgaard; J M Christensen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  442     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-10-27     Completed Date:  1988-10-27     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  325-32     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chloral Hydrate / analysis
Chromatography, Gas
Decarboxylation
Hydrogen-Ion Concentration
Liver / analysis*,  metabolism
Male
Rats
Rats, Inbred Strains
Trichloroethylene / analysis*
Chemical
Reg. No./Substance:
290YE8AR51/Trichloroethylene; 418M5916WG/Chloral Hydrate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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