| Determination of tributyrin and its metabolite butyrate in Wistar rat plasma samples by gas chromatography/mass spectrometry. | |
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MedLine Citation:
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PMID: 15384139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A gas chromatographic (GC) method with mass spectrometric (MS) detection was developed for the determination of tributyrin and its metabolite butyrate in rat plasma. Following precipitation of plasma protein with acetonitrile, the analytes in the samples were separated on a DB-5ms capillary column with helium as carrier gas. Phenylmethylsulfonyl fluoride (PMSF), an inhibitor for serine proteases, papain and acetylcholinesterase, was found to be essential to inhibit the activity of enzyme(s) responsible for the hydrolysis of tributyrin in both rat and human blood samples. The enzyme inhibitor in 5 mM (final concentration) was added immediately into the blood samples after collection to prevent the hydrolysis. The linear concentration ranges for tributyrin and butyrate were 0.1-2.0 and 1-20 microM, respectively. The coefficients of variation for intra-day and inter-day assays for tributyrin were all <10%, and those for butyrate were also <10%, except for the lowest concentration (1 microM), which was less than 20%. The accuracy of all concentration determinations ranged from 96.0-110.0%. The limit of quantification (LOQ) was 0.1 microM for tributyrin and 1.0 microM for butyrate. This method could detect tributyrin and butyrate simultaneously, and represents an improvement in sensitivity for the detection of tributyrin compared with the previous gas chromatography-flame ionization detection (GC-FID) method. |
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Authors:
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Jie Su; Ningning Zhang; Paul C Ho |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Validation Studies |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 18 ISSN: 0951-4198 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2004 |
Date Detail:
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Created Date: 2004-09-22 Completed Date: 2004-11-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 2217-22 Citation Subset: IM |
Copyright Information:
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Copyright 2004 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Pharmacy, Faculty of Science, National University of Singapore, 18 Science Drive 4, Singapore 117543. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral Animals Blood Chemical Analysis / methods* Butyrates / blood*, chemistry Gas Chromatography-Mass Spectrometry / methods* Male Phenylmethylsulfonyl Fluoride / chemistry Rats Rats, Wistar Reproducibility of Results Sensitivity and Specificity Triglycerides / administration & dosage, blood*, chemistry Tumor Markers, Biological / blood* |
| Chemical | |
Reg. No./Substance:
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0/Butyrates; 0/Triglycerides; 0/Tumor Markers, Biological; 329-98-6/Phenylmethylsulfonyl Fluoride; 60-01-5/tributyrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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