Document Detail


Determination of the transiently lowered pKa of the retinal Schiff base during the photocycle of bacteriorhodopsin.
MedLine Citation:
PMID:  8643698     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reprotonation of the transiently deprotonated retinal Schiff base in the bacteriorhodopsin photocycle is greatly slowed when the proton donor Asp-96 is removed with site-specific mutagenesis, but its rate is restored upon adding azide or other weak acids such as formate and cyanate. As expected, between pH 3 and 7 the rate of Schiff base protonation in the photocycle of the D96N mutant correlates with the concentrations of the acid forms of these agents. Dissection of the rates in the biexponential reprotonation kinetics of the Schiff base between pH 7 and 9 yielded calculated rate constants for the protonation equilibrium. Their dependencies on pH and azide or cyanate concentrations are consistent with both earlier suggested mechanisms: (i) azide and other weak acids may function as proton carriers in the protonation equilibrium of the Schiff base, or (ii) the binding of their anionic forms may catalyze proton conduction to and from the Schiff base. The measured rate constants allow the calculation of the pKa of the Schiff base during its reprotonation in the photocycle of D96N. It is 8.2-8.3, a value much below the pKa determined earlier in unphotolyzed bacteriorhodopsin.
Authors:
L S Brown; J K Lanyi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  93     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-07-17     Completed Date:  1996-07-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1731-4     Citation Subset:  IM; S    
Affiliation:
Department of Physiology and Biophysics, University of California, Irvine, 92717, USA.
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MeSH Terms
Descriptor/Qualifier:
Azides / pharmacology
Bacteriorhodopsins / genetics,  metabolism,  radiation effects*
Cyanates / pharmacology
Formic Acids / pharmacology
Hydrogen-Ion Concentration
Kinetics
Models, Chemical
Photolysis
Protons
Schiff Bases / metabolism
Grant Support
ID/Acronym/Agency:
GM29498/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Azides; 0/Cyanates; 0/Formic Acids; 0/Protons; 0/Schiff Bases; 53026-44-1/Bacteriorhodopsins; 64-18-6/formic acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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