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Determination of the transient period of the EIS complex and investigation of the suppression of blood glucose levels by L: -arabinose in healthy adults.
MedLine Citation:
PMID:  21165628     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
PURPOSE: L: -Arabinose uncompetitively inhibits intestinal sucrase by forming an enzyme-inhibitor-substrate (EIS) complex. The transient period of the EIS complex affects the time span of inhibition. We determined the apparent transient period of the EIS complex of sucrase, L: -arabinose, and sucrose both in vitro and in humans. METHODS: Intestinal acetone powder (a source of sucrase), L: -arabinose, and sucrose were mixed and injected into a dialysis membrane that was placed in a sucrose solution. The production rate of D: -glucose and the release rate of L: -arabinose from sucrase were determined. We also investigated the suppression of blood glucose levels by L: -arabinose in 21 healthy volunteers. Sucrose (40 g) was ingested with or without L: -arabinose (2 g), then blood glucose values were measured, which returned to steady-state conditions within 2 h. Volunteers were then given 90 g of commercial adzuki bean jelly containing 40 g sucrose as the sucrose load, and blood glucose values were measured again. RESULTS: Addition of L: -arabinose reduced the production rate of D: -glucose compared to the rates measured in the absence of L: -arabinose for several hours in vitro. L: -Arabinose was released at a lower rate in the presence of sucrose than in its absence. Blood glucose values measured 2 h after sucrose was given with L: -arabinose were significantly lower than those measured when L: -arabinose was not given (Δ change in maximum value: with L: -arabinose, 53.8 ± 19.7 mg/dL; without L: -arabinose, 65.0 ± 17.7 mg/dL). CONCLUSION: The EIS complex of sucrase-L: -arabinose-sucrose was maintained for several hours both in vitro and in humans.
Authors:
Kiyoshi Shibanuma; Yoko Degawa; Koichi Houda
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-17
Journal Detail:
Title:  European journal of nutrition     Volume:  -     ISSN:  1436-6215     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888704     Medline TA:  Eur J Nutr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Research & Development Laboratory, Sanwa Cornstarch Co. Ltd., 594 Unate, Kashihara-shi, Nara Pref, 634-8585, Japan, kiyoshi-shibanuma@sanwa-starch.co.jp.
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