| Determination of the transient period of the EIS complex and investigation of the suppression of blood glucose levels by L: -arabinose in healthy adults. | |
MedLine Citation:
|
PMID: 21165628 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
PURPOSE: L: -Arabinose uncompetitively inhibits intestinal sucrase by forming an enzyme-inhibitor-substrate (EIS) complex. The transient period of the EIS complex affects the time span of inhibition. We determined the apparent transient period of the EIS complex of sucrase, L: -arabinose, and sucrose both in vitro and in humans. METHODS: Intestinal acetone powder (a source of sucrase), L: -arabinose, and sucrose were mixed and injected into a dialysis membrane that was placed in a sucrose solution. The production rate of D: -glucose and the release rate of L: -arabinose from sucrase were determined. We also investigated the suppression of blood glucose levels by L: -arabinose in 21 healthy volunteers. Sucrose (40 g) was ingested with or without L: -arabinose (2 g), then blood glucose values were measured, which returned to steady-state conditions within 2 h. Volunteers were then given 90 g of commercial adzuki bean jelly containing 40 g sucrose as the sucrose load, and blood glucose values were measured again. RESULTS: Addition of L: -arabinose reduced the production rate of D: -glucose compared to the rates measured in the absence of L: -arabinose for several hours in vitro. L: -Arabinose was released at a lower rate in the presence of sucrose than in its absence. Blood glucose values measured 2 h after sucrose was given with L: -arabinose were significantly lower than those measured when L: -arabinose was not given (Δ change in maximum value: with L: -arabinose, 53.8 ± 19.7 mg/dL; without L: -arabinose, 65.0 ± 17.7 mg/dL). CONCLUSION: The EIS complex of sucrase-L: -arabinose-sucrose was maintained for several hours both in vitro and in humans. |
Authors:
|
Kiyoshi Shibanuma; Yoko Degawa; Koichi Houda |
Related Documents
:
|
16032158 - Relationship between blood selenium concentration or glutathione peroxidase activity, a... 20723698 - Effect of method of conservation of timothy on endogenous nitrogen flows in lactating d... 1887368 - Radioprotection of whole-body gamma-irradiation-induced alteration in some haematologic... 11219438 - Effectiveness of gnrh plus prostaglandin f2alpha for estrus synchronization in cattle o... 17337288 - Ionizing radiation induces upregulation of cellular procoagulability and tissue factor ... 6441898 - Blood flow compensates oxygen demand in the vulnerable ca3 region of the hippocampus du... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2010-12-17 |
Journal Detail:
|
Title: European journal of nutrition Volume: - ISSN: 1436-6215 ISO Abbreviation: - Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-12-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100888704 Medline TA: Eur J Nutr Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Research & Development Laboratory, Sanwa Cornstarch Co. Ltd., 594 Unate, Kashihara-shi, Nara Pref, 634-8585, Japan, kiyoshi-shibanuma@sanwa-starch.co.jp. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transcatheter aortic valve implantation: evidence on safety and efficacy compared with medical thera...
Next Document: Short-term isocaloric manipulation of carbohydrate intake: effect on subsequent ad libitum energy in...