Document Detail


Determination of trace levels of fatty acid metal salts by high-performance liquid chromatography with fluorescence prelabeling.
MedLine Citation:
PMID:  6721136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple method is described for picomole determinations of fatty acid metal salts. Fatty acid salts are directly labeled with 4-bromomethyl-7-methoxycoumarin in the presence of excess ethylenediaminetetraacetic acid tripotassium salt without any solvent extractions. The fluorescence derivatives of fatty acids are separated by reverse-phase high-performance liquid chromatography followed by fluorometric detection. The response of each fatty acid (C8-C18) calcium salt is linear from 1 to 50 micrograms/ml of samples. The detection limit is about 7 pmol. Good recoveries are obtained for the calcium salts of myrystic acid and soap (C8-C18, C18:1,2). The new method is successfully applied to the study on biodegradation of fatty acids in river water.
Authors:
K Hayashi; J Kawase; K Yoshimura; K Ara; K Tsuji
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  136     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1984 Feb 
Date Detail:
Created Date:  1984-05-30     Completed Date:  1984-05-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  314-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biodegradation, Environmental
Calcium / analysis
Chromatography, High Pressure Liquid*
Fatty Acids / analysis*
Fluorescence
Fluorometry
Fresh Water / analysis*
Salts / analysis*
Soaps / analysis
Umbelliferones
Water / analysis*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Salts; 0/Soaps; 0/Umbelliferones; 35231-44-8/4-bromomethyl-7-methoxycoumarin; 7440-70-2/Calcium; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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