Document Detail

Determination of total selenium and selenium distribution in the milk phases in commercial cow's milk by HG-AAS.
MedLine Citation:
PMID:  15761740     Owner:  NLM     Status:  MEDLINE    
A procedure has been developed for determining the selenium in cow's milk using hydride generation-atomic absorption spectrometry (HG-AAS) following microwave-assisted acid digestion. The selenium distributions in milk whey, fat and micellar casein phases were studied after separating the different phases by ultracentrifugation and determining the selenium in all of them. The detection limits obtained by HG-AAS for the whole milk, milk whey and micellar casein were 0.074, 0.065 and 0.075 microg l(-1), respectively. The accuracy for the whole milk was checked by using a Certified Reference Material CRM 8435 whole milk powder from NIST, and the analytical recoveries for the milk whey and casein micelles were 100.9 and 96.9%, respectively. A mass balance study of the determination of selenium in the different milk phases was carried out, obtaining values of 95.5-100.8%. The total content of selenium was determined in 37 milk samples from 15 different manufacturers, 19 whole milk samples and 18 skimmed milk samples. The selenium levels found were within the 8.5-21 microg l(-1) range. The selenium distributions in the different milk phases were studied in 14 whole milk samples, and the highest selenium levels were found in milk whey (47.2-73.6%), while the lowest level was found for the fat phase (4.8-16.2%). A strong correlation was found between the selenium levels in whole milk and the selenium levels in the milk components.
Oscar Muñiz-Naveiro; Raquel Domínguez-González; Adela Bermejo-Barrera; José A Cocho; José M Fraga; Pilar Bermejo-Barrera
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-03-11
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  381     ISSN:  1618-2642     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-25     Completed Date:  2005-09-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1145-51     Citation Subset:  IM    
Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avda. de las Ciencias s/n, 15782, Santiago de Compostela, Spain.
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MeSH Terms
Fats / chemistry
Food Analysis*
Milk / chemistry*
Selenium / analysis*
Spectrophotometry, Atomic / methods*
Time Factors
Reg. No./Substance:
0/Fats; 7782-49-2/Selenium

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