Document Detail

Determination of tobacco alkaloids in single plant cells by capillary electrophoresis.
MedLine Citation:
PMID:  11403483     Owner:  NLM     Status:  MEDLINE    
A new capillary electrophoresis system with direct UV detection for the analysis of the tobacco alkaloids nicotine, nornicotine and anabasine in plant microsamples was developed. An electrolyte containing a high concentration of citric acid to provide good buffer capacity at pH 3.6 was found to be most suitable in terms of sensitivity and separation efficiency. At this low pH the tobacco alkaloids are present in cationic form, showing high mobility and increased UV absorption. This system was used for the analysis of nicotine in single epidermal leaf cells of tobacco plants. Only vacuolar concentrations of nicotine were determined, as the vacuole occupies >95% of the entire volume in epidermal cells. The procedure of sample acquisition and preparation for nicotine analysis of vacuolar samples in the pl range is shown. The results indicate a gradient of nicotine from the leaf base to the tip with higher concentrations present in the cells at the tip. Compared to simultaneously measured bulk leaf samples containing all types of cells, tissues and compartments, the concentrations in epidermal cells are much higher. As nicotine is the major defence substance against insects in tobacco and the epidermis is the most exposed leaf tissue this result is physiologically plausible.
H Lochmann; A Bazzanella; S Kropsch; K Bächmann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  917     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-06-13     Completed Date:  2001-10-25     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  311-7     Citation Subset:  IM    
Department of Chemistry, Institute of Inorganic Chemistry, Darmstadt University of Technology, Germany.
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MeSH Terms
Electrophoresis, Capillary / methods*
Nicotine / analysis*
Plants, Toxic*
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Tobacco / chemistry*,  cytology
Reg. No./Substance:

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