| Determination of thiopental in urine sample with high-performance liquid chromatography using iodine-azide reaction as a postcolumn detection system. | |
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MedLine Citation:
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PMID: 16112624 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The reaction between iodine and azide ions induced by thiopental was utilized as a postcolumn reaction for chromatographic determination of thiopental. The method is based on the separation of thiopental on an Nova-Pak CN HP column with an acetonitrile-aqueous solution of sodium azide as a mobile phase, followed by spectrophotometric measurement of the residual iodine (lambda=350 nm) from the postcolumn iodine-azide reaction induced by thiopental after mixing an iodine solution containing iodide ions with the column effluent containing azide ions and thiopental. Chromatograms obtained for thiopental showed negative peaks as a result of the decrease in background absorbance. The detection limit (defined as S/N=3) was 20 nM (0.4 pmol injected amount) for thiopental. Calibration graphs, plotted as peak area versus concentrations, were linear from 40 nM. The elaborated method was applied to determine thiopental in urine samples. The detection limit (defined as S/N=3) was 0.025 nmol/ml urine. Calibration graphs, plotted as peak area versus concentrations, were linear from 0.05 nmol/ml urine. Authentic urine samples were analyzed, thiopental was determined at nmol/ml urine level. |
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Authors:
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Robert Zakrzewski; Witold Ciesielski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 824 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2005-08-30 Completed Date: 2005-10-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 327-32 Citation Subset: IM |
Affiliation:
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Department of Instrumental Analysis, University of Łódź, Pomorska 163, 90-236 Łódź, Poland. robzak@chemul.uni.lodz.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetonitriles
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chemistry Anesthetics, Intravenous / urine* Azides / chemistry Chromatography, High Pressure Liquid / instrumentation, methods* Female Humans Hydrogen-Ion Concentration Iodine / chemistry Male Mass Spectrometry / methods Reproducibility of Results Sodium Chloride / chemistry Temperature Thiopental / chemistry, urine* |
| Chemical | |
Reg. No./Substance:
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0/Acetonitriles; 0/Anesthetics, Intravenous; 0/Azides; 75-05-8/acetonitrile; 7553-56-2/Iodine; 76-75-5/Thiopental; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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