| Determination of theanine, GABA, and other amino acids in green, oolong, black, and Pu-erh teas with dabsylation and high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 18652476 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dabsyl chloride (dimethylaminoazobenzene sulfonyl chloride), a useful chromophoric labeling reagent for amino acids and amines, was developed in this laboratory in 1975. Although several methods have been developed to determine various types of amino acids, a quick and easy method of determining theanine, GABA, and other amino acids has not been developed in one HPLC system. In this paper are analyzed the free amino acid contents of theanine and GABA in different teas (green tea, black tea, oolong tea, Pu-erh tea, and GABA tea) with a dabsylation and reverse phase high-performance liquid chromatography (HPLC) system coupled with a detector at 425 nm absorbance. Two reverse phase columns, Hypersil GOLD and Zorbax ODS, were used and gave different resolutions of dabsyl amino acids in the gradient elution program. The data suggest that the tea source or the steps of tea-making may contribute to the theanine contents variations. High theanine contents of high-mountain tea were observed in both green tea and oolong tea. Furthermore, the raw (natural fermented) Pu-erh tea contained more theanine than ripe (wet fermented) Pu-erh tea, and the GABA contents in normal teas were generally lower than that in GABA tea. |
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Authors:
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Kai-Yang Syu; Chih-Li Lin; Hsiu-Chen Huang; Jen-Kun Lin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-25 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 56 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-03 Completed Date: 2008-10-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 7637-43 Citation Subset: IM |
Affiliation:
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Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Chromatography, High Pressure Liquid* Glutamates / analysis* Indicators and Reagents Tea / chemistry* gamma-Aminobutyric Acid / analysis* p-Dimethylaminoazobenzene / analogs & derivatives* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Glutamates; 0/Indicators and Reagents; 0/Tea; 3081-61-6/theanine; 56-12-2/gamma-Aminobutyric Acid; 56512-49-3/4-N,N-dimethylaminoazobenzene-4'-sulfonyl chloride; 60-11-7/p-Dimethylaminoazobenzene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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