Document Detail


Determination of testosterone:epitestosterone ratio after pentafluorophenyldimethylsilyl-trimethylsilyl derivatisation using gas chromatography-mass spectrometry in equine urine.
MedLine Citation:
PMID:  10616737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A highly specific method is described for measuring the testosterone:epitestosterone ratio in equine urine by gas chromatography-mass spectrometry (GC-MS) with stable isotope internal standards. The procedure was based on Serdolit Pad-1 resin extraction, enzymatic hydrolysis, and chemical derivatisation prior to instrumental analysis. The mixed derivatives, 3-trimethylsilyl-17-pentafluorophenyldimethylsilyl ether (3-TMS-17-flophemesyl) testosterone and epitestosterone, were found to have excellent analytical properties. The specificity of the derivatisation method exploits a unique feature of steroids: the selective exchange of the alcoholic flophemesyl ether for the trimethylsilyl ether. The sensitivity and specificity of the mixed 3-TMS-17-flophemesyl derivatives allow adequate determinations of testosterone and epitestosterone, even in urine from mares, in 5 ml samples. The repeatability of testosterone and epitestosterone was 6.2 and 5.7%, respectively, and their reproducibility was in the range of 6.4-8.7%.
Authors:
M H Choi; J Y Kim; B C Chung
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Analyst     Volume:  124     ISSN:  0003-2654     ISO Abbreviation:  Analyst     Publication Date:  1999 May 
Date Detail:
Created Date:  2000-01-24     Completed Date:  2000-01-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  675-8     Citation Subset:  IM    
Affiliation:
Doping Control Center, Korea Institute of Science and Technology, Cheongryang, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Epitestosterone / urine
Gas Chromatography-Mass Spectrometry / methods
Horses / urine*
Male
Testosterone / urine*
Chemical
Reg. No./Substance:
481-30-1/Epitestosterone; 58-22-0/Testosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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