| Determination of testicular function after torsion by DNA flow cytometry of serial fine-needle aspirates. | |
| | |
MedLine Citation:
|
PMID: 9117230 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To determine the efficacy of DNA flow cytometric analysis of testicular percutaneous fine-needle aspirates in the assessment and follow-up of testicular function after torsion, and to determine the relationship between the duration of torsion and testicular injury. MATERIALS AND METHODS: Three groups of 15 adult rats underwent a 720 degrees torsion, with fixation of the mesorchial ligament, for 1, 3 or 5 h. Bilateral aspirations, performed 7, 21 and 35 days after torsion were examined by flow cytometry. Testes were harvested and evaluated histologically using Johnsen's scoring. RESULTS: Irreversible testicular injury occurred in all three groups of rats, with loss of function after 1 h and loss of viability after 3 and 5 h. The results from flow cytometry suggested significant contralateral testicular injury (P < 0.025) but this was not supported by the histological evaluation. There was a strong correlation between the testicular function assessed by flow cytometry and by Johnsen's scoring of histological specimens (r2 = 0.95). CONCLUSION: The assessment of testicular aspirates by flow cytometry allows testicular function to be followed after torsion in rats, and potentially in humans. Using DNA flow cytometry, the temporal course of the twisted testis in the adult rat was determined; contralateral testicular injury following the reversal of torsion could not be excluded. |
| | |
Authors:
|
T A Carroll; M C Regan; R Alyusuf; D Greene; B Curran; E Kay; M Leader; J M Fitzpatrick |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: British journal of urology Volume: 79 ISSN: 0007-1331 ISO Abbreviation: Br J Urol Publication Date: 1997 Mar |
Date Detail:
|
Created Date: 1997-04-24 Completed Date: 1997-04-24 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 15740090R Medline TA: Br J Urol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 449-54 Citation Subset: IM |
Affiliation:
|
Department of Surgery, Mater Misericordiae Hospital, University College Dublin, Ireland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biopsy, Needle DNA / analysis* Flow Cytometry Male Necrosis Ploidies Rats Rats, Sprague-Dawley Spermatic Cord Torsion / genetics, pathology*, physiopathology Spermatogenesis |
| Chemical | |
Reg. No./Substance:
|
9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Assessment of testicular cytology by fine-needle aspiration and the imprint technique: are they reli...
Next Document: A new approach using tissue alkaline phosphatase activity to identify early testicular cancer.