| Determination of tenuazonic acid in human urine by means of a stable isotope dilution assay. | |
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MedLine Citation:
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PMID: 23397093 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The content of tenuazonic acid in human urine was determined by a stable isotope dilution assay (SIDA) that was recently developed for the analysis of food commodities and extensively re-validated for urine matrix in this study. Linearity of the response curve was proven between molar ratios n(labeled standard)/n(analyte) of 0.02-100. The limits of detection and determination were 0.2 and 0.6 μg/L, respectively. The mean recovery of the stable isotope dilution assay was 102 ± 3 % in the range between 1.0 and 100 μg/L. Interassay precision was 6.7 % (relative standard deviation of three triplicate analyses of a human urine sample during 3 weeks). The method was applied to two studies dealing with urinary excretion of tenuazonic acid: In the first study, tenuazonic acid was quantified in the 24-h urine of six volunteers from Germany (three female, three male) in a concentration range of 1.3-17.3 μg/L or 2.3-10.3 ng/mg(-1) creatinine, respectively. In the second study, two volunteers (one female, one male) ingested 30 μg tenuazonic acid by consumption of naturally contaminated whole meal sorghum infant cereals and tomato juice, respectively. The urinary excretion of the ingested tenuazonic acid was 54-81 % after 6 h, depending on matrix and volunteer. After 24 h, 87-93 % of the ingested amount of tenuazonic acid was excreted, but the fate of the remaining about 10 % is open. Thus, it is not possible to exclude potential health hazards for the consumer, completely. |
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Authors:
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Stefan Asam; Katharina Habler; Michael Rychlik |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-2-9 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: - ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-2-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Lehrstuhl für Analytische Lebensmittelchemie, Technische Universität München, Alte Akademie 10, 85354, Freising, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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