Document Detail


Determination of subunit composition of Clostridium cellulovorans cellulosomes that degrade plant cell walls.
MedLine Citation:
PMID:  11916675     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Clostridium cellulovorans produces a cellulase enzyme complex (cellulosome). In this study, we isolated two plant cell wall-degrading cellulosomal fractions from culture supernatant of C. cellulovorans and determined their subunit compositions and enzymatic activities. One of the cellulosomal fractions showed fourfold-higher plant cell wall-degrading activity than the other. Both cellulosomal fractions contained the same nine subunits (the scaffolding protein CbpA, endoglucanases EngE and EngK, cellobiohydrolase ExgS, xylanase XynA, mannanase ManA, and three unknown proteins), although the relative amounts of the subunits differed. Since only cellobiose was released from plant cell walls by the cellulosomal fractions, cellobiohydrolases were considered to be key enzymes for plant cell wall degradation.
Authors:
Koichiro Murashima; Akihiko Kosugi; Roy H Doi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-03-27     Completed Date:  2002-07-12     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1610-5     Citation Subset:  IM    
Affiliation:
Section of Molecular, University of California, Davis, California 95616, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Blotting, Western
Cell Wall / metabolism*
Cellulase / chemistry*,  metabolism
Cellulose 1,4-beta-Cellobiosidase
Clostridium / enzymology*,  growth & development
Culture Media
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Multienzyme Complexes / chemistry*,  metabolism
Plant Structures / metabolism*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Multienzyme Complexes; EC 3.2.1.4/Cellulase; EC 3.2.1.91/Cellulose 1,4-beta-Cellobiosidase
Comments/Corrections

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