Document Detail


Determination of subcellular concentrations of soluble carbohydrates in rose petals during opening by nonaqueous fractionation method combined with infiltration-centrifugation method.
MedLine Citation:
PMID:  20183924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Petal growth associated with flower opening depends on cell expansion. To understand the role of soluble carbohydrates in petal cell expansion during flower opening, changes in soluble carbohydrate concentrations in vacuole, cytoplasm and apoplast of petal cells during flower opening in rose (Rosa hybrida L.) were investigated. We determined the subcellular distribution of soluble carbohydrates by combining nonaqueous fractionation method and infiltration-centrifugation method. During petal growth, fructose and glucose rapidly accumulated in the vacuole, reaching a maximum when petals almost reflected. Transmission electron microscopy showed that the volume of vacuole and air space drastically increased with petal growth. Carbohydrate concentration was calculated for each compartment of the petal cells and in petals that almost reflected, glucose and fructose concentrations increased to higher than 100 mM in the vacuole. Osmotic pressure increased in apoplast and symplast during flower opening, and this increase was mainly attributed to increases in fructose and glucose concentrations. No large difference in osmotic pressure due to soluble carbohydrates was observed between the apoplast and symplast before flower opening, but total osmotic pressure was much higher in the symplast than in the apoplast, a difference that was partially attributed to inorganic ions. An increase in osmotic pressure due to the continued accumulation of glucose and fructose in the symplast may facilitate water influx into cells, contributing to cell expansion associated with flower opening under conditions where osmotic pressure is higher in the symplast than in the apoplast.
Authors:
Kunio Yamada; Ryo Norikoshi; Katsumi Suzuki; Hideo Imanishi; Kazuo Ichimura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Planta     Volume:  230     ISSN:  1432-2048     ISO Abbreviation:  Planta     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2010-02-24     Completed Date:  2010-05-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1115-27     Citation Subset:  IM    
Affiliation:
National Institute of Floricultural Science, Fujimoto, Tsukuba, Ibaraki 305-8519, Japan.
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Anions / analysis
Carbohydrates / analysis*,  chemistry
Cations / analysis
Cell Fractionation / methods
Cell Wall / chemistry
Centrifugation / methods
Chromatography, High Pressure Liquid
Cytoplasm / chemistry
Flowers / chemistry*,  growth & development,  ultrastructure
Fructose / analysis
Glucose / analysis
Microscopy, Electron, Transmission
Osmotic Pressure
Rosa / chemistry*,  cytology,  growth & development
Solubility
Subcellular Fractions / chemistry
Sucrose / analysis
Time Factors
Vacuoles / chemistry
Chemical
Reg. No./Substance:
0/Anions; 0/Carbohydrates; 0/Cations; 30237-26-4/Fructose; 50-99-7/Glucose; 57-50-1/Sucrose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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