| Determination of some hydroxycholesterols in human serum samples. | |
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MedLine Citation:
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PMID: 3597624 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The simultaneous determination of some hydroxycholesterols in human serum samples is described. The procedure is based on hydrolysis and extraction of these compounds in serum samples, followed by removal of especially cholesterol (making use of reversed-phase high-performance liquid chromatography) and derivatization of the purified compounds to their trimethylsilyl ethers and subsequent gas chromatography using flame ionization detection. Serum levels of 7 alpha-hydroxycholesterol, 7 beta-hydroxycholesterol and 26-hydroxycholesterol were determined in several groups of patients: normals, untreated patients suffering from cerebrotendinous xanthomatosis, patients suffering from cerebrotendinous xanthomatosis and treated with either chenodeoxycholic acid or cholic acid in an effective dose, patients suffering from cerebro-hepato-renal syndrome, patients suffering from hypercholesterolemia and treated with cholestyramine for prolonged periods and one patient presumed to be suffering from an inborn error of metabolism in bile acid synthesis. It can be concluded that the 7 alpha-hydroxycholesterol concentration in serum is a good parameter for establishing disorders involving the metabolic conversion of 7 alpha-hydroxycholesterol towards bile acids. In addition, 26-hydroxycholesterol levels in patients suffering from cerebrotendinous xanthomatosis are beyond detectable limits, even during treatment with bile acids in an effective dose, whereas in all other conditions this compound is substantially present. |
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Authors:
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B J Koopman; J C van der Molen; B G Wolthers; J B Vanderpas |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 416 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1987 Apr |
Date Detail:
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Created Date: 1987-08-07 Completed Date: 1987-08-07 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 1-13 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Gas Humans Hydroxycholesterols / blood* Xanthomatosis / blood |
| Chemical | |
Reg. No./Substance:
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0/Hydroxycholesterols; 13095-61-9/cholest-5-ene-3 beta,26-diol; 566-26-7/cholest-5-en-3 beta,7 alpha-diol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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