| Determination of sialic acid by the thiobarbituric acid reaction in sweet whey and its fractions. | |
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MedLine Citation:
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PMID: 10552534 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Determination of sialic acid in sweet whey is useful as the concentration of sialic acid reflects the amount of glycomacropeptide (GMP) present. In this study, the concentration of total sialic acid was determined by the thiobarbituric acid reaction after dialysis of samples in water, and the concentration of GMP sialic acid was estimated by gel chromatography on Sephacryl S-200. Concentrations of total and GMP sialic acid determined in a sweet whey sample prepared from fresh milk were 2.0 and 1.5 microg/mg of dry weight, respectively. Analysis of commercial samples showed that the concentration of total sialic acid in sweet whey was 9 times lower than that in whey protein concentrate but 18 times higher than that in whey permeate. A similar trend was observed in the variation of GMP sialic acid concentration between sweet whey and why protein concentrate. The concentration of sialic acid differed 10 times between two samples of whey protein isolate. |
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Authors:
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T Nakano; L Ozimek |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 47 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 1999 Jul |
Date Detail:
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Created Date: 2000-08-30 Completed Date: 2000-08-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2613-6 Citation Subset: IM |
Affiliation:
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Alberta Dairy Association Research Unit, c/o Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Gel Chromatography, Ion Exchange Dairy Products / analysis* N-Acetylneuraminic Acid / analysis* Thiobarbiturates / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Thiobarbiturates; 131-48-6/N-Acetylneuraminic Acid; 504-17-6/thiobarbituric acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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