Document Detail


Determination of short-chain free fatty acids in lipolyzed milk fat by capillary electrophoresis.
MedLine Citation:
PMID:  10797874     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to monitor the release of short-chain free fatty acids (FFA) from milk fat during hydrolysis with lipase using capillary electrophoresis. Sample and run buffer allowed FFA to be maintained in solution by using cyclodextrin and methanol. Indirect UV detection at 270 nm was used, employing p-anisate as a chromophore. Calibration curves constructed for each individual FFA followed linear relationships with highly significant (p < 0.01) correlation coefficients. Electrophoretic FFA profiles of fresh milk fat and lipolyzed milk fat showed marked qualitative and quantitative differences. Butanoic acid (C4) was found in a concentration of 64 ppm, while hexanoic (C6) and octanoic (C8) acids were found in concentrations of 3.8 ppm in fresh milk fat. After a 60-min hydrolysis with commercial lipase, FFA released from milk fat consisted mainly of high concentrations (ppm) of butanoic (C4) (900), followed by hexanoic (C6) (427), octanoic (C8) (282), decanoic (C10) (92), pentanoic (C5) (47), and dodecanoic (C12) (37.5) acids. Ratios of FFA that were associated with flavor balance were calculated. The application of CE for lipolysis monitoring in milk fat offers a simple and fast method for the determination of FFA. Quantitative data can be obtained in 20 min, including sample preparation. The lengthy and laborious steps required in traditional chromatographic techniques, such as lipid extraction, FFA isolation, and derivatization, were not required in this CE method. The implementation of CE for milk fat lipolysis monitoring may be a useful quality control tool for dairy flavor development and production.
Authors:
B Vallejo-Cordoba; M A Mazorra-Manzano; A F González-Córdova
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of capillary electrophoresis     Volume:  5     ISSN:  1079-5383     ISO Abbreviation:  J Capillary Electrophor     Publication Date:    1998 May-Aug
Date Detail:
Created Date:  2000-06-08     Completed Date:  2000-06-08     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9507610     Medline TA:  J Capillary Electrophor     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  111-4     Citation Subset:  IM    
Affiliation:
Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Hermosillo, Sonora, México.
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MeSH Terms
Descriptor/Qualifier:
Animals
Electrophoresis, Capillary / methods
Fats / metabolism*
Fatty Acids, Nonesterified / analysis*
Lipolysis
Milk / metabolism*
Chemical
Reg. No./Substance:
0/Fats; 0/Fatty Acids, Nonesterified

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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