| Determination of riboflavin and flavocoenzymes in human blood plasma by high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 8546438 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A high-performance liquid chromatographic method for determining riboflavin, flavin adenine dinucleotide, and flavin mononucleotide in human blood plasma is presented. Flavocoenzymes are determined as flavin mononucleotide after acid hydrolysis of flavin adenine dinucleotide. Metabolites are separated by reversed-phase column chromatography and quantified by their native fluorescence. Criteria of quality are (riboflavin/flavocoenzymes): coefficients of variation 2.8/4.6% (intra-assay) and 2.8/4.4% (inter-assay); recovery 82.4/94.4%; detection limit < 3.0/9.0 nmol/l. Because sample preparation requires only few steps, and the retention times are short ( < 5 min), this method is recommended for use in routine analysis. |
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Authors:
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J Zempleni |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Annals of nutrition & metabolism Volume: 39 ISSN: 0250-6807 ISO Abbreviation: Ann. Nutr. Metab. Publication Date: 1995 |
Date Detail:
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Created Date: 1996-02-14 Completed Date: 1996-02-14 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8105511 Medline TA: Ann Nutr Metab Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 224-6 Citation Subset: IM |
Affiliation:
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Institute of Nutritional Sciences, Justus Liebig University, Giessen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid Flavin Mononucleotide / blood* Flavin-Adenine Dinucleotide / blood* Humans Riboflavin / blood* |
| Chemical | |
Reg. No./Substance:
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146-14-5/Flavin-Adenine Dinucleotide; 146-17-8/Flavin Mononucleotide; 83-88-5/Riboflavin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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