Document Detail


Determination of riboflavin and flavocoenzymes in human blood plasma by high-performance liquid chromatography.
MedLine Citation:
PMID:  8546438     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A high-performance liquid chromatographic method for determining riboflavin, flavin adenine dinucleotide, and flavin mononucleotide in human blood plasma is presented. Flavocoenzymes are determined as flavin mononucleotide after acid hydrolysis of flavin adenine dinucleotide. Metabolites are separated by reversed-phase column chromatography and quantified by their native fluorescence. Criteria of quality are (riboflavin/flavocoenzymes): coefficients of variation 2.8/4.6% (intra-assay) and 2.8/4.4% (inter-assay); recovery 82.4/94.4%; detection limit < 3.0/9.0 nmol/l. Because sample preparation requires only few steps, and the retention times are short ( < 5 min), this method is recommended for use in routine analysis.
Authors:
J Zempleni
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of nutrition & metabolism     Volume:  39     ISSN:  0250-6807     ISO Abbreviation:  Ann. Nutr. Metab.     Publication Date:  1995  
Date Detail:
Created Date:  1996-02-14     Completed Date:  1996-02-14     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8105511     Medline TA:  Ann Nutr Metab     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  224-6     Citation Subset:  IM    
Affiliation:
Institute of Nutritional Sciences, Justus Liebig University, Giessen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Flavin Mononucleotide / blood*
Flavin-Adenine Dinucleotide / blood*
Humans
Riboflavin / blood*
Chemical
Reg. No./Substance:
146-14-5/Flavin-Adenine Dinucleotide; 146-17-8/Flavin Mononucleotide; 83-88-5/Riboflavin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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