Document Detail


Determination of relative protein degradation activity at different life stages in rainbow trout (Oncorhynchus mykiss).
MedLine Citation:
PMID:  19038357     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rainbow trout were reared from 5 g to approximately 400 g on a diet formulated to supply the required protein from either fishmeal or plant proteins. The fish were sampled at every weight doubling and liver and muscle samples were obtained. From these tissue samples RNA and protein were isolated and analyzed for the expression of a number of muscle regulatory and protein degradation genes and enzymatic activity for proteins involved in the caspase, calpain, and ubiquitin-proteasome pathways for protein proteolysis. Only MyoD2 showed significant differences in expression between the two diets, while no significant changes over the course of the experiment were determined for MyoD2 or the other muscle factors. For the degradation genes significant changes in expression were determined for calpain1 and calpastatin. Calpastatin also showed a significant increase in expression over the course of the experiment in the muscle of fish fed a fishmeal diet and significant decrease in expression in the liver of fish fed the fishmeal based diet. Differences in proteasome enzyme activity were found between diets in the liver and muscle of fish and for caspase-3 activity in muscle. Significant changes in activity over the course of the experiment were noted for proteasome and calpain activity in the liver and muscle. These findings suggest that diets replacing fishmeal with plant material can have some effects on protein turnover in muscle and that some degradation pathways are differentially regulated during the growth of rainbow trout.
Authors:
Ken Overturf; T Gibson Gaylord
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Publication Detail:
Type:  Journal Article     Date:  2008-11-06
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  152     ISSN:  1879-1107     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-09     Completed Date:  2009-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  150-60     Citation Subset:  IM    
Affiliation:
USDA-ARS, Hagerman Fish Culture Experiment Station, Hagerman, ID 83332, USA. Ken.Overturf@ars.usda.gov
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Weight / drug effects
Diet
Feeding Behavior / drug effects
Fish Proteins / metabolism*
Gene Expression Regulation, Developmental / drug effects
Life Cycle Stages* / drug effects
Liver / drug effects,  enzymology
Muscle Proteins / genetics,  metabolism
Muscles / drug effects,  enzymology
Oncorhynchus mykiss / growth & development*,  metabolism*
Plant Proteins / administration & dosage,  pharmacology
Protein Processing, Post-Translational* / drug effects,  genetics
Chemical
Reg. No./Substance:
0/Fish Proteins; 0/Muscle Proteins; 0/Plant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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