Document Detail


Determination of regions important for monoamine oxidase (MAO) A and B substrate and inhibitor selectivities.
MedLine Citation:
PMID:  9564602     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MAO-A and -B are defined by their substrate and inhibitor preferences. To determine which regions of the isoenzymes confer these preferences, we have constructed six chimeric MAO enzymes by reciprocally exchanging corresponding N-terminal, C-terminal, and internal segments of MAO-A and -B then determined the catalytic properties of these chimeric enzymes. N-terminal chimerics A45B and B36A were made by exchanging amino acid segments 1-45 and 1-36 of MAO-A and -B respectively. C-terminal chimerics A402B and B393A were made by exchanging amino acid segments 403-527 and 394-520 of MAO-A and -B respectively, and internal chimerics AB161-375A and BA152-366B were made by exchanging amino acid segments 161-375 and 152-366 of MAO-A and -B respectively. The enzymatic properties observed for the chimerics suggest that the exchanged internal regions but not the N- or C-terminal regions confer substrate and inhibitor preferences.
Authors:
J C Shih; K Chen; R M Geha
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neural transmission. Supplementum     Volume:  52     ISSN:  0303-6995     ISO Abbreviation:  J. Neural Transm. Suppl.     Publication Date:  1998  
Date Detail:
Created Date:  1998-06-10     Completed Date:  1998-06-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0425126     Medline TA:  J Neural Transm Suppl     Country:  AUSTRIA    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Affiliation:
Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, Los Angeles, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
COS Cells
Clorgyline / pharmacology
Humans
Isoenzymes / chemistry*,  metabolism*
Kinetics
Monoamine Oxidase / chemistry*,  metabolism*
Monoamine Oxidase Inhibitors / pharmacology*
Mutagenesis, Site-Directed
Point Mutation
Recombinant Fusion Proteins / chemistry,  metabolism
Selegiline / pharmacology
Sequence Homology, Amino Acid
Transfection
Grant Support
ID/Acronym/Agency:
KO5 MH00796/MH/NIMH NIH HHS; R01 MH37020/MH/NIMH NIH HHS; R37 MH39085/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/Monoamine Oxidase Inhibitors; 0/Recombinant Fusion Proteins; 14611-51-9/Selegiline; 17780-72-2/Clorgyline; EC 1.4.3.4/Monoamine Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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