Document Detail


Determination of reference values for a novel ketoamine-specific fructosamine assay for assessment of diabetic glycemic control.
MedLine Citation:
PMID:  11474831     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Intensive monitoring to improve glycemic control is essential for effective management of diabetes and reduction of long-term morbidity and pathology. Measurement of glycated serum proteins (fructosamine) allows more frequent assessment (monthly) of glycemic control than the 2- to 3-month window of the traditional glycated hemoglobin (HbA1c) assay. In response to concerns about assays designed to measure glycated serum proteins based on the nitroblue tetrazolium (NBT) methodology, a novel assay to measure glycated serum proteins has been developed based on the specific oxidation of the ketoamine bonds within the glycated proteins. METHODS: Reference range values for this new, enzymatic glycated-serum-protein assay (GlyPro; Genzyme Corporation, Cambridge, MA) are reported. RESULTS: The GlyPro reference range is lower and shows close correlation with ranges reported for the NBT assay. The 95% overall reference range was 122 to 236 mumol/L. CONCLUSIONS: GlyPro is a reliable, accurate assay and correlates well with the NBT assay for the measurement of glycated serum proteins. The assay may be useful in the short-term assessment of diabetes control, a necessary complement to long-term control as assessed by hemoglobin A1c (HbA1c) assays.
Authors:
J E O'Brien; M Brookes
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diabetes technology & therapeutics     Volume:  1     ISSN:  1520-9156     ISO Abbreviation:  Diabetes Technol. Ther.     Publication Date:  1999  
Date Detail:
Created Date:  2001-07-27     Completed Date:  2001-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100889084     Medline TA:  Diabetes Technol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  447-55     Citation Subset:  IM    
Affiliation:
Quest Diagnostics Incorporated, Teterboro, New Jersey, USA. obrienj@questdiagnostics.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Markers / blood
Blood Glucose / metabolism*
Diabetes Mellitus / blood*
Female
Fructosamine / blood*
Hemoglobin A, Glycosylated / analysis
Humans
Male
Middle Aged
Monitoring, Physiologic / methods
Reference Standards
Reference Values
Regression Analysis
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Blood Glucose; 0/Hemoglobin A, Glycosylated; 4429-04-3/Fructosamine
Comments/Corrections
Comment In:
Diabetes Technol Ther. 1999 Winter;1(4):457-9   [PMID:  11474832 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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