Document Detail

Determination of the rate of true fertility in duck breeds by the combination of two in vitro methods.
MedLine Citation:
PMID:  15168754     Owner:  NLM     Status:  MEDLINE    
Embryonic mortality is a significant problem plaguing the hatching success. Its early forms are especially hardly distinguishable from true infertility. Propidium iodide (PI) staining of the germinal disc combined with outer perivitelline layer (OPVL) sperm counting was used for the determination of 'true' fertility of duck eggs in two different experiments: fertility investigation on fresh, unincubated eggs of Hungarian ducks and on incubated eggs of a crossbred, selected as 'infertile' at the 7th day of incubation. Examination of the relationship between OPVL sperm count and fertility seems to be an adequate tool for checking the effectiveness of insemination programmes and the fertilising capacity of poultry spermatozoa. The proportion of fertile eggs was around 50% when the number of OPVL sperm was between 0.1 and 0.2 spermatozoa/mm2. Ninety-nine percent of the eggs containing > 0.3 OPVL sperm/mm2 were fertile and all of the eggs containing < 0.05 sperm/mm2 were infertile. To assure the accuracy of fertility prediction by OPVL sperm counting, PI staining of the germinal disc was used to determine fertility in uncertain cases. Identification of very early embryonic mortality, i.e. that occurring before oviposition, is very difficult. The use of a dissecting microscope for the assessment of real fertility is suitable in most of the cases, while PI staining of the germinal discs proved to be more reliable for detecting very early embryonic death. The combination of the two methods proved to be a useful tool for detecting the 'true' fertility of duck eggs of different breeds.
Krisztina Liptói; A Varga; A Hidas; Judit Barna
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Acta veterinaria Hungarica     Volume:  52     ISSN:  0236-6290     ISO Abbreviation:  Acta Vet. Hung.     Publication Date:  2004  
Date Detail:
Created Date:  2004-05-31     Completed Date:  2004-06-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406376     Medline TA:  Acta Vet Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  227-33     Citation Subset:  IM    
Institute for Small Animal Research, H-2100 Gödöllo, PO Box 417, Hungary.
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MeSH Terms
Ovum / physiology*
Sperm Count / veterinary*
Staining and Labeling / veterinary
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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