| Determination of rat plasma levels of sertraline enantiomers using direct injection with achiral-chiral column switching by LC-ESI/MS/MS. | |
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MedLine Citation:
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PMID: 19959312 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A highly sensitive and selective on-line two-dimensional reversed-phase liquid chromatography/electrospray ionization-tandem mass spectrometric (2D-LC-ESI/MS/MS) method to determine sertraline (SRT) enantiomers in rat plasma was developed and validated. The method was applied to separate and determine the diastereomers and enantiomers of SRT simultaneously. The 2D-LC-ESI/MS/MS system consisted of RAM column in first dimension for trapping proteineous part of plasma and a chiral Cyclobond column as second dimension for separation of enantiomers and diastereomers of SRT using 0.1% aqueous trifluoroacetic acid:acetonitrile (86:14, v/v) as mobile phase in an isocratic elution mode. The linear dynamic range was 0.5-200ng/mL (r(2)>0.999). Acceptable precision and accuracy were obtained over the calibration range. The assay was successfully used in the analysis of SRT enantiomers in rat plasma to support pharmacokinetic studies. |
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Authors:
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R Nageswara Rao; K Nagesh Kumar; Dhananjay D Shinde |
Publication Detail:
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Type: Journal Article Date: 2009-09-25 |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 52 ISSN: 1873-264X ISO Abbreviation: J Pharm Biomed Anal Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-03-08 Completed Date: 2010-05-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: England |
Other Details:
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Languages: eng Pagination: 398-405 Citation Subset: IM |
Copyright Information:
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Copyright 2009. Published by Elsevier B.V. |
Affiliation:
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Analytical Chemistry Division, Discovery Laboratory, Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500607, India. rnrao55@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calibration Chromatography, Liquid / methods* Male Quality Control Rats Rats, Wistar Reference Standards Reproducibility of Results Sensitivity and Specificity Serotonin Uptake Inhibitors / blood* Sertraline / blood* Spectrometry, Mass, Electrospray Ionization / methods* Stereoisomerism Tandem Mass Spectrometry / methods* |
| Chemical | |
Reg. No./Substance:
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0/Serotonin Uptake Inhibitors; 79617-96-2/Sertraline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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