Document Detail


Determination of purines including 2,8-dihydroxyadenine in urine using capillary electrophoresis.
MedLine Citation:
PMID:  11100858     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
For the purpose of rapid drug monitoring, methods have been developed for the determination of 2,8-dihydroxyadenine, allopurinol, oxypurinol, adenine, hypoxanthine, hippuric acid and xanthine in urine with and without sodium dodecyl sulfate as additive in sodium tetraborate running buffer. No sample preparation is necessary. 6-methylmercaptopurine and etofylline have been used as the internal standards. The limit of detection is 5 microM and the range of quantification stretches from 20 to 2000 microM. The capillary electrophoresis methods are simple, fast and robust.
Authors:
T Wessel; C Lanvers; S Fruend; G Hempel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  894     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2001-02-20     Completed Date:  2001-03-08     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  157-64     Citation Subset:  IM    
Affiliation:
Abt Hämatologie/Onkologie, Universiäts-Kinderklinik, Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adenine / analogs & derivatives*,  urine*
Electrophoresis, Capillary / methods*
Humans
Purines / analysis*
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Purines; 30377-37-8/2,8-dihydroxyadenine; 73-24-5/Adenine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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