Document Detail

Determination of the profile of fatty acids of 4 species of Shigella spp by chromatography of gases.
MedLine Citation:
PMID:  17063774     Owner:  NLM     Status:  MEDLINE    
Shigella boydii, Shigella flexneri, Shigella dysenteriae and Shigella sonnei were identified using gas chromatography instead of the traditional techniques. Their acid methyl esters profiles were determined using a gas chromatograph Hewlett Packard 5890A and a RSL-150 heliflex capillary column. A total of 192 samples were analyzed both reference strains (ATCC 8700, INDRE B2188, B2194 and B2199) and environmental isolates. 12 fatty acids were included in the profiles from which 3-hydroxytetradecanoic acid (peak 12), trans 9-octadecanoic acid (peak 22), heptadecanoic acid (peak 18) and octadecanoic acid (peak 23), were the most important for the differentiation of the species analyzed.
E Robles Valderrama; M D Hurtado Bocanegra; M E González Arreaga; P Ramírez García; A Durán Díaz; R Ayala Patiño; M E Martínez Pérez
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Revista latinoamericana de microbiología     Volume:  44     ISSN:  0187-4640     ISO Abbreviation:  Rev. Latinoam. Microbiol.     Publication Date:    2002 Apr-Jun
Date Detail:
Created Date:  2006-10-26     Completed Date:  2007-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0242625     Medline TA:  Rev Latinoam Microbiol     Country:  Mexico    
Other Details:
Languages:  eng     Pagination:  69-74     Citation Subset:  IM    
Unidad de Investigación Interdisciplinaria en Ciencias de la Salud y la Educación, UNAM FES Iztacala, México.
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MeSH Terms
Chromatography, Gas
Fatty Acids / analysis*
Shigella / chemistry*
Reg. No./Substance:
0/Fatty Acids

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