Document Detail


Determination of primary structure of two isoforms 6-1 and 6-2 PLA2 D49 from Bothrops jararacussu snake venom and neurotoxic characterization using in vitro neuromuscular preparation.
MedLine Citation:
PMID:  16862457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper we reported the purification, the biological characterization and the amino acid sequence of two new isoforms basic 6-1 (Bj-IV) and 6-2 (Bj-V) PLA(2) D49 purified from the Bothrops jararacussu venom. The isoforms 6-1 and 6-2 had a sequence of amino acids of 121 amino acid residues 6-1: DLFEWGQMIL KETGKNPFPY YGAYGCYCGW GGRGKPKDKD TDRCCYVHDC CYKKLTGCPK TDDRYSYSWL DLTIVCGEDD PCKELCECDK AIAVCFRENL GTYNKKYRYH LKPCKKADKP C and pI value 7.83 and 6-2: DLWQFGQMIL KETGKIPFPY YGAYGCYCGW GGRGGKPKDG TDRCCYVHDC CYKKLTGCPK TDDRYSYSWL DLTIVCGEDD PCKELCECDK AIAVCFRENL GTYNKKYRYH LKPCKKADKP C with a pI value of 7.99. Skeletal muscle preparations from the young chicken have been used previously in order to study the effects of toxins on neuromuscular transmission, providing an important opportunity to study the differentiated behavior of a toxin before more than one model, because it shows differences in its sensibilities. Both isoforms have produced neuromuscular blockade in young chicken biventer cervicis nerve-muscle preparations in presence or absence of crotapotin crotalic (F3 and F4) indicating that catalytic activity was not essential for neuromuscular action in this preparation.
Authors:
L A Ponce-Soto; V L Bonfim; L Rodrigues-Simioni; J C Novello; S Marangoni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The protein journal     Volume:  25     ISSN:  1572-3887     ISO Abbreviation:  Protein J.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-07-24     Completed Date:  2007-01-26     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  101212092     Medline TA:  Protein J     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  147-55     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Institute of Biology, State University of Campinas, SP, Brazil. poncesoto@yahoo.com.ar
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Bothrops*
Chickens
Crotalid Venoms / enzymology*,  toxicity
Crotoxin / pharmacology
Isoenzymes / chemistry,  isolation & purification,  metabolism,  toxicity
Molecular Sequence Data
Neuromuscular Junction / drug effects*
Phospholipases A / chemistry*,  isolation & purification,  metabolism,  toxicity*
Phospholipases A2
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/Crotalid Venoms; 0/Isoenzymes; 9007-40-3/Crotoxin; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

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