| Determination of pools of tricarboxylic acid cycle and related acids in bacteria. | |
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MedLine Citation:
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PMID: 337897 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Methods for sampling, extracting, and quantitating the metabolic pools of organic acids from bacteria have been developed. The concentration of these metabolites was determined by a new gas chromatographic method that can quantitatively determine the levels of lactate, pyruvate, fumarate, succinate, malate, alpha-ketoglutarate, and citrate. Values obtained were confirmed by fluorimetric analyses of five of the individual acids. In Escherichia coli, pools range from about 1 to 5 mumol/g of dry weight, with a variation in replicate samples of 5 to 15%. Under similar conditions, these pools in Bacillus licheniformis are in the same range, although the pyruvic acid pool is significantly larger. |
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Authors:
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W H Siegel; T Donohue; R W Bernlohr |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 34 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 1977 Nov |
Date Detail:
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Created Date: 1978-01-27 Completed Date: 1978-01-27 Revised Date: 2010-09-02 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 512-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus
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analysis*,
metabolism Carboxylic Acids / analysis* Chromatography, Gas Citrates / analysis Citric Acid Cycle Escherichia coli / analysis*, metabolism Fluorometry Fumarates / analysis Glucose / metabolism Glutamates / metabolism Ketoglutaric Acids / analysis Lactates / analysis Malates / analysis Pyruvates / analysis Succinates / analysis |
| Chemical | |
Reg. No./Substance:
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0/Carboxylic Acids; 0/Citrates; 0/Fumarates; 0/Glutamates; 0/Ketoglutaric Acids; 0/Lactates; 0/Malates; 0/Pyruvates; 0/Succinates; 50-99-7/Glucose |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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