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Determination of polyamines in human plasma by high-performance liquid chromatography coupled with Q-TOF mass spectrometry.
MedLine Citation:
PMID:  23019166     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A high-performance liquid chromatography coupled with Q-time of flight mass spectrometry (HPLC/Q-TOF MS) method was developed and validated for the determination of 1, 3-diaminopropane, putrescine, cadaverine, spermidine and spermine in human plasma. The plasma samples were first pretreated by 10% HClO(4) and then derived by benzoyl chloride with 1, 6-diaminohexane as internal standard. The derived polyamines were separated on a C(18) column using a gradient program. The detection was performed on a Q-TOF MS by positive ionization mode. Calibration curve for each polyamine was obtained in the concentration range of 0.4 ~ 200.0 ng • ml(-1) , with limit of detection of 0.02 ~ 0.1 ng • ml(-1) . The intra- and inter-day RSD for all polyamines were 2.5-14.0% and 2.9 ~ 13.4%, respectively. The method was applied to determine the polyamines in human plasma from cancer patients and healthy volunteers. Results showed that the mean levels of polyamines in the plasma of cancer patients were higher than that of healthy volunteers, which suggested that the plasma polyamines could be employed as cancer diagnostic indicators in clinical testing. Copyright © 2012 John Wiley & Sons, Ltd.
Authors:
Ran Liu; Kaishun Bi; Ying Jia; Qian Wang; Ran Yin; Qing Li
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of mass spectrometry : JMS     Volume:  47     ISSN:  1096-9888     ISO Abbreviation:  J Mass Spectrom     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9504818     Medline TA:  J Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  1341-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Affiliation:
School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China.
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