Document Detail

Determination of plasma lipid hydroperoxides by an NADPH/NADP+ coupled enzyme reaction system. Evaluation of a method.
MedLine Citation:
PMID:  9476616     Owner:  NLM     Status:  MEDLINE    
Several techniques based on different principles have been proposed to measure lipid hydroperoxides. Enzymatic methods are sensitive and can be quite specific but they are subject to interference by inhibitors and not all are stoichiometric. The present work proposes some modifications of the Heath & Tappel (Anal Biochem 1976; 7:184-91) enzymatic method of determination of lipid hydroperoxides in order to standardize and automate it and to meet the analytical criteria required for a biological assay. The proposed new protocol and the automated assay give acceptable within-run and between-run precisions, with coefficients of variation of 3.34% and 5.80%, respectively, at the usual plasma lipid hydroperoxides content. The recovery is 98.7 +/- 4.89%, and the method is linear for a wide range of contents and sensitive (10 mumol/l) enough to measure the plasma lipid hydroperoxides content.
C Ruiz; A Alegría; R Barberá; R Farré; M J Lagarda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies     Volume:  35     ISSN:  0939-4974     ISO Abbreviation:  Eur J Clin Chem Clin Biochem     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-04-03     Completed Date:  1998-04-03     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9105775     Medline TA:  Eur J Clin Chem Clin Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  893-8     Citation Subset:  IM    
Departamento de Medicina Preventiva y Salud Pública, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Valencia, Spain.
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MeSH Terms
Glutathione Peroxidase / metabolism
Glutathione Reductase / metabolism
Lipid Peroxides / blood*
NADP / metabolism
Reproducibility of Results
Spectrophotometry / methods*
Reg. No./Substance:
0/Lipid Peroxides; 53-59-8/NADP; EC Peroxidase; EC Reductase

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