Document Detail

Determination of plasma amino acids by fluorescent derivatization and reversed-phase liquid chromatographic separation.
MedLine Citation:
PMID:  15074468     Owner:  NLM     Status:  MEDLINE    
Amino acid analysis in physiological fluids remains expensive, as it usually requires a dedicated analyzer. We modified an RP-HPLC method originally devoted to peptide and protein hydrolysate analysis in order to apply it to plasma amino acid determination. The described method uses a commercial kit based on 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate derivatization followed by reversed-phase high-performance liquid chromatography. The described method is easy to use and allows the determination of twelve amino acids frequently involved in aminoacidopathies, thus enabling the diagnosis and follow-up of affected patients. The results obtained by this method are comparable to those obtained with a classical amino acid analyzer.
Stephanie Badiou; Sylvain Lehmann; Jean-Paul Cristol; Helene Bellet
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical laboratory     Volume:  50     ISSN:  1433-6510     ISO Abbreviation:  Clin. Lab.     Publication Date:  2004  
Date Detail:
Created Date:  2004-04-12     Completed Date:  2004-10-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9705611     Medline TA:  Clin Lab     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  153-8     Citation Subset:  IM    
Laboratoire de Biochimie, Hôpital Lapeyronie, Montpellier, France.
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MeSH Terms
Amino Acids / blood*,  chemistry
Aminoquinolines / chemistry
Carbamates / chemistry
Chromatography, High Pressure Liquid / methods*
Fluorescent Dyes* / chemistry
Metabolism, Inborn Errors / diagnosis
Reagent Kits, Diagnostic
Reproducibility of Results
Reg. No./Substance:
0/6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; 0/Amino Acids; 0/Aminoquinolines; 0/Carbamates; 0/Fluorescent Dyes; 0/Reagent Kits, Diagnostic

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