| Determination of phenolic acids using Trametes versicolor laccase. | |
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MedLine Citation:
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PMID: 19071305 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Two biosensors based on Trametes versicolor laccase (TvL) were developed for the determination of phenolic compounds. Commercial oxygen electrode and ferrocene-modified screen-printed graphite electrodes were used for preparation of laccase biosensors. The systems were calibrated for three phenolic acids. Linearity was obtained in the concentration range 0.1-1.0muM caffeic acid, 0.05-0.2muM ferulic acid, 2.0-14.0muM syringic acid for laccase immobilised on a commercial oxygen electrode and 2.0-30.0muM caffeic acid, 2.0-10.0muM ferulic acid, 4.0-30.0muM syringic acid for laccase immobilised on ferrocene-modified screen-printed electrodes. Furthermore, optimal pH, temperature and thermal stability studies were performed with the commercial oxygen electrode. Both electrodes were used for determination of a class of phenolic acids, achieving a cheap and fast tool and an easy to be used procedure for screening real samples of human plasma. |
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Authors:
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Dilek Odaci; Suna Timur; Nurdan Pazarlioglu; Maria Rita Montereali; Walter Vastarella; Roberto Pilloton; Azmi Telefoncu |
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Publication Detail:
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Type: Journal Article Date: 2006-06-12 |
Journal Detail:
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Title: Talanta Volume: 71 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2008-12-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984816R Medline TA: Talanta Country: England |
Other Details:
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Languages: eng Pagination: 312-7 Citation Subset: - |
Affiliation:
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Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova-Izmir, Turkey. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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