Document Detail


Determination of phenolic acids using Trametes versicolor laccase.
MedLine Citation:
PMID:  19071305     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Two biosensors based on Trametes versicolor laccase (TvL) were developed for the determination of phenolic compounds. Commercial oxygen electrode and ferrocene-modified screen-printed graphite electrodes were used for preparation of laccase biosensors. The systems were calibrated for three phenolic acids. Linearity was obtained in the concentration range 0.1-1.0muM caffeic acid, 0.05-0.2muM ferulic acid, 2.0-14.0muM syringic acid for laccase immobilised on a commercial oxygen electrode and 2.0-30.0muM caffeic acid, 2.0-10.0muM ferulic acid, 4.0-30.0muM syringic acid for laccase immobilised on ferrocene-modified screen-printed electrodes. Furthermore, optimal pH, temperature and thermal stability studies were performed with the commercial oxygen electrode. Both electrodes were used for determination of a class of phenolic acids, achieving a cheap and fast tool and an easy to be used procedure for screening real samples of human plasma.
Authors:
Dilek Odaci; Suna Timur; Nurdan Pazarlioglu; Maria Rita Montereali; Walter Vastarella; Roberto Pilloton; Azmi Telefoncu
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Publication Detail:
Type:  Journal Article     Date:  2006-06-12
Journal Detail:
Title:  Talanta     Volume:  71     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2008-12-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  312-7     Citation Subset:  -    
Affiliation:
Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova-Izmir, Turkey.
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