Document Detail


Determination and pharmacokinetics of orientin in rabbit plasma by liquid chromatography after intravenous administration of orientin and Trollius chinensis Bunge extract.
MedLine Citation:
PMID:  17416555     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A high-performance liquid chromatography (HPLC) method was developed and validated for the determination of orientin in rabbit plasma using ultraviolet (UV) absorbance detection. Orientin is the active constituent of purified herbal extract (TRO PE) from the flower of Trollius chinensis Bunge. Protein precipitation was used as the sample preparation technique. A Diamonsil C18 column (150 mmx4.6 mm, 5 microm) was equilibrated with a mobile phase composed of 0.1% acetic acid/methanol/acetonitrile (80/5/15, v/v/v). The calibration curve of orientin in rabbit plasma was linear in the concentration range of 0.530-53.0 microg/mL. This validated method was successfully applied to a pharmacokinetic study in rabbits after the intravenous administrations of orientin and TRO PE at three different doses.
Authors:
Xiaoqin Li; Taoguang Huo; Feng Qin; Xiumei Lu; Famei Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-25
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  853     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-15     Completed Date:  2007-08-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  221-6     Citation Subset:  IM    
Affiliation:
Department of Analytical Chemistry, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Liquid / methods*
Flavonoids / administration & dosage,  blood*,  pharmacokinetics*
Glucosides / administration & dosage,  blood*,  pharmacokinetics*
Injections, Intravenous
Plant Extracts / administration & dosage,  blood,  pharmacokinetics
Rabbits
Ranunculaceae / chemistry*
Reproducibility of Results
Chemical
Reg. No./Substance:
0/Flavonoids; 0/Glucosides; 0/Plant Extracts; 28608-75-5/orientin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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