Document Detail


Determination of patulin in apple juice by liquid chromatography.
MedLine Citation:
PMID:  16512238     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method was developed and validated in-house for the determination of patulin (PAT), a toxic mold metabolite, in apple juice. The sample was extracted with ethyl acetate-hexane and analyzed by liquid chromatography equipped with a C18 column and diode array detector. The mobile phase used for the quantification was water-ethanol, at a flow rate of 0.5 mL/min. The method showed a mean recovery of 84.8%, the relative standard deviation obtained in the precision study was <7.7%, the quantification and detection limits were 7 and 3 microg/L, respectively, and the linear range for PAT in apple juice was 2.6-650 microg/L. The ruggedness was evaluated by an intralaboratory experiment, in which 5 factors were studied, and only one was found to influence the observed results. The developed method is fast, practical, and simple; the solvents (except hexane) and reagents used were nontoxic. The results of the validation confirmed the efficiency of the method, which is sensitive enough to be used in studies required to quantify PAT in apple juice.
Authors:
Maria Helena Iha; Myrna Sabino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of AOAC International     Volume:  89     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2006 Jan-Feb
Date Detail:
Created Date:  2006-03-03     Completed Date:  2006-05-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  139-43     Citation Subset:  IM    
Affiliation:
Instituto Adolfo Lutz - Laboratório I de Ribeirão Preto, Rua Minas, 877, CEP 14085-410, Ribeirão Preto-SP, Brazil. mhiha@ial.sp.gov.br
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MeSH Terms
Descriptor/Qualifier:
Chemistry Techniques, Analytical / methods*
Chromatography, Liquid / methods*
Ethanol / chemistry
Food Analysis / methods*
Food Contamination
Hexanes / chemistry
Malus / metabolism*
Patulin / analysis*,  chemistry
Reproducibility of Results
Solvents / chemistry
Spectrophotometry
Time Factors
Chemical
Reg. No./Substance:
0/Hexanes; 0/Solvents; 149-29-1/Patulin; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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