| Determination of patulin in apple juice by liquid chromatography. | |
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MedLine Citation:
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PMID: 16512238 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method was developed and validated in-house for the determination of patulin (PAT), a toxic mold metabolite, in apple juice. The sample was extracted with ethyl acetate-hexane and analyzed by liquid chromatography equipped with a C18 column and diode array detector. The mobile phase used for the quantification was water-ethanol, at a flow rate of 0.5 mL/min. The method showed a mean recovery of 84.8%, the relative standard deviation obtained in the precision study was <7.7%, the quantification and detection limits were 7 and 3 microg/L, respectively, and the linear range for PAT in apple juice was 2.6-650 microg/L. The ruggedness was evaluated by an intralaboratory experiment, in which 5 factors were studied, and only one was found to influence the observed results. The developed method is fast, practical, and simple; the solvents (except hexane) and reagents used were nontoxic. The results of the validation confirmed the efficiency of the method, which is sensitive enough to be used in studies required to quantify PAT in apple juice. |
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Authors:
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Maria Helena Iha; Myrna Sabino |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of AOAC International Volume: 89 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2006 Jan-Feb |
Date Detail:
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Created Date: 2006-03-03 Completed Date: 2006-05-23 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 139-43 Citation Subset: IM |
Affiliation:
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Instituto Adolfo Lutz - Laboratório I de Ribeirão Preto, Rua Minas, 877, CEP 14085-410, Ribeirão Preto-SP, Brazil. mhiha@ial.sp.gov.br |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chemistry Techniques, Analytical
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methods* Chromatography, Liquid / methods* Ethanol / chemistry Food Analysis / methods* Food Contamination Hexanes / chemistry Malus / metabolism* Patulin / analysis*, chemistry Reproducibility of Results Solvents / chemistry Spectrophotometry Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Hexanes; 0/Solvents; 149-29-1/Patulin; 64-17-5/Ethanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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