Document Detail


Determination of pantothenic acid in infant milk formulas by high performance liquid chromatography.
MedLine Citation:
PMID:  8744215     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A reverse-phase liquid chromatographic method was adapted for the assay of pantothenic acid in infant milk formulas. Sample preparation consisted of deproteination with acetic acid and sodium acetate solutions, followed by centrifugation and filtration. The chromatographic system included a C-18 column and a mobile phase consisting of a sodium phosphate buffer and acetonitrile (97:3, vol/vol). The column effluent was monitored by UV detection at 197 nm. The system was linear from 50 to 800 ng. The recoveries of pantothenic acid from augmented samples ranged from 89 to 98%, and the coefficients of variation ranged from 1.17 to 3.20%. The results obtained with the HPLC and a microbiological method were highly correlated for starting infant formula, follow-up infant formula, and formula for infants of low birth weight from four different manufactures. All formulas analyzed contained pantothenic acid at concentrations higher than those declared on their nutritional labels and were in compliance with international recommendations.
Authors:
J M Romera; M Ramirez; A Gil
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of dairy science     Volume:  79     ISSN:  0022-0302     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-10-17     Completed Date:  1996-10-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  523-6     Citation Subset:  IM    
Affiliation:
Abbott Laboratories S.A., Research and Development Department, Granada, Spain.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid / methods*,  statistics & numerical data
Drug Stability
Food Labeling
Humans
Infant
Infant Food / analysis*
Pantothenic Acid / analysis*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
79-83-4/Pantothenic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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