Document Detail


Determination of the pK for the acid-induced denaturation of ferrocytochrome c.
MedLine Citation:
PMID:  15035626     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Optical absorption spectroscopy was used to characterize the acid-induced conformational transition of horse heart ferrocytochrome c in the presence of urea. By using linear extrapolation to zero denaturant concentration, an apparent pK value for denaturation was found to be 0.86 +/- 0.07 at 25 degrees C. Visible absorption spectra in the presence of high urea concentration indicate that the dominant population is a high-spin, five-coordinate form under acidic conditions. Ferricytochrome c, used as a model reference system, shows a linear dependence of pK values versus urea concentration in the range from 0 to 4.1 M. Our data also indicate that even at a pH below 2 the iron-sulfur bond in ferrocytochrome c is present.
Authors:
Rastislav Varhac; Marián Antalík
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry     Volume:  43     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-23     Completed Date:  2004-07-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3564-9     Citation Subset:  IM    
Affiliation:
Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 043 53 Kosice, Slovakia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cytochrome c Group / chemistry*
Ferric Compounds / chemistry
Ferrous Compounds / chemistry
Horses
Hydrochloric Acid / chemistry*
Hydrogen-Ion Concentration
Myocardium / enzymology
Oxidation-Reduction
Protein Conformation
Protein Denaturation
Protein Folding
Spectrophotometry, Ultraviolet
Urea
Chemical
Reg. No./Substance:
0/Cytochrome c Group; 0/Ferric Compounds; 0/Ferrous Compounds; 0/cytochrome c553; 57-13-6/Urea; 7647-01-0/Hydrochloric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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