Document Detail

Determination of organophosphorus pesticide residues in vegetables by an enzyme inhibition method using α-naphthyl acetate esterase extracted from wheat flour.
MedLine Citation:
PMID:  22467368     Owner:  NLM     Status:  MEDLINE    
The widespread use of organophosphorus pesticides (OPs) poses a great threat to human health and has made the detection of OP residues in food an important task, especially in view of the fact that easy and rapid detection methods are needed. Because OPs have inhibitory effects on the activity of α-naphthyl acetate esterase (ANAE) in plants, in this work we evaluated the possibility of detecting OPs in vegetables with ANAE extracted from commercial flour. The limits of detection (LODs) obtained for methamidophos, dichlorvos, phoxim, dimethoate, and malathion in lettuce samples with crude ANAE were 0.17, 0.11, 0.11, 0.96, and 1.70 mg/kg, respectively. Based on the maximum residue limits (MRLs) for OPs in food stipulated by Chinese laws which are 0.05, 0.20, 0.05, 1.00, and 8.00 mg/kg for methamidophos, dichlorvos, phoxim, dimethoate, and malathion, respectively, the esterase inhibition method with crude ANAE had sufficient sensitivity to detect the residues of dichlorvos, dimethoate, and malathion in lettuce, but it could not be used to guarantee the safety of the same samples if methamidophos or phoxim residue was present. The sensitivity of the method was improved by the use of esterase purified by ammonium sulfate salting-out. The LODs obtained for methamidophos and phoxim with purified esterase were lower than the MRLs for these OPs in food. This is a very promising method for the detection of OP residues in vegetables using crude or purified esterase because of its cheapness, sensitivity, and convenience.
Jun-liang Wang; Qing Xia; An-ping Zhang; Xiao-yan Hu; Chun-mian Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of Zhejiang University. Science. B     Volume:  13     ISSN:  1862-1783     ISO Abbreviation:  J Zhejiang Univ Sci B     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-02     Completed Date:  2012-08-07     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101236535     Medline TA:  J Zhejiang Univ Sci B     Country:  China    
Other Details:
Languages:  eng     Pagination:  267-73     Citation Subset:  IM    
International Joint Research Center for Persistent Toxic Substances, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China.
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MeSH Terms
Food Analysis / methods*
Food Contamination / analysis*
Naphthol AS D Esterase / chemistry*
Organophosphorus Compounds / analysis*,  chemistry
Pesticides / analysis*,  chemistry
Plant Extracts / chemistry
Triticum / enzymology*
Vegetables / chemistry*
Reg. No./Substance:
0/Organophosphorus Compounds; 0/Pesticides; 0/Plant Extracts; EC 3.1.-/Naphthol AS D Esterase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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