Document Detail

Determination of organic acids in seven wheat varieties by capillary gas chromatography.
MedLine Citation:
PMID:  4073499     Owner:  NLM     Status:  MEDLINE    
Fresh wheat tops were extracted with acidic 90% ethanol, and the ethanol was evaporated and a portion of the aqueous residue loaded onto DEAE-Sephadex. Organic acids were eluted with pyridinium formate and then lyophilized and the dried residue was derivatized with 1% trimethylchlorosilane in bis(trimethylsilyl)trifluoroacetamide. The acids were then quantitatively determined using capillary gas chromatography and identified using capillary gas chromatography-mass spectrometry. The acidic ethanol extraction of fresh plant tissue was quantitative for all acids except citric while losses in the remaining procedures were controlled by using an internal standard. The ion exchange chromatography made the greatest contribution to experimental error, imposing a minimum loading requirement of 0.1 mumol of each acid for adequate precision. Organic acid profiles were determined for seven wheat cultivars (Triticum aestivum cv Carazinho, Teal, Lance, Warigal, Isis, Maringa, and BH1146) grown on gravel in solution culture for 30 days. Profiles were simple, consisting of only malic, aconitic, and citric acids, with levels of each acid for all varieties falling within the range 2-5 mumol/g fresh tissue. Storage of samples led to a large increase in sampling error and increased the amount of extractable citric acid.
D G Burke; E Hilliard; K Watkins; V Russ; B J Scott
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  149     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1986-01-08     Completed Date:  1986-01-08     Revised Date:  2002-11-01    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  421-9     Citation Subset:  IM    
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MeSH Terms
Carboxylic Acids / analysis*
Chromatography, Gas
Chromatography, Ion Exchange
Citric Acid Cycle
Species Specificity
Trimethylsilyl Compounds
Triticum / analysis*
Reg. No./Substance:
0/Carboxylic Acids; 0/Trimethylsilyl Compounds; 21149-38-2/N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide

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