Document Detail


Determination of organic acids in seven wheat varieties by capillary gas chromatography.
MedLine Citation:
PMID:  4073499     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fresh wheat tops were extracted with acidic 90% ethanol, and the ethanol was evaporated and a portion of the aqueous residue loaded onto DEAE-Sephadex. Organic acids were eluted with pyridinium formate and then lyophilized and the dried residue was derivatized with 1% trimethylchlorosilane in bis(trimethylsilyl)trifluoroacetamide. The acids were then quantitatively determined using capillary gas chromatography and identified using capillary gas chromatography-mass spectrometry. The acidic ethanol extraction of fresh plant tissue was quantitative for all acids except citric while losses in the remaining procedures were controlled by using an internal standard. The ion exchange chromatography made the greatest contribution to experimental error, imposing a minimum loading requirement of 0.1 mumol of each acid for adequate precision. Organic acid profiles were determined for seven wheat cultivars (Triticum aestivum cv Carazinho, Teal, Lance, Warigal, Isis, Maringa, and BH1146) grown on gravel in solution culture for 30 days. Profiles were simple, consisting of only malic, aconitic, and citric acids, with levels of each acid for all varieties falling within the range 2-5 mumol/g fresh tissue. Storage of samples led to a large increase in sampling error and increased the amount of extractable citric acid.
Authors:
D G Burke; E Hilliard; K Watkins; V Russ; B J Scott
Related Documents :
1819339 - The lactic acid and citric acid content in the gingival fluid of orthodontic patients.
193739 - Integration of lipid utilization with krebs cycle activity in muscle.
12005349 - Endothelin-1 in citric acid aerosol inhalation-induced airway constriction of guinea pigs.
9195009 - Physiological characterization of a microbial sensor containing the yeast arxula adenin...
15641759 - Assessment of enamel erosion and protective effect of salivary pellicle by surface roug...
9423149 - Properties of citric acid at the glass transition.
3754209 - Evaluation of the renal scanning agent n-succinyldesferrioxamine b in dogs.
18961269 - Sulpholane as a solvent for titration of barbiturates and sulpha drugs.
18343209 - Prenatal diagnosis of propionic acidemia by measuring methylcitric acid in dried amniot...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  149     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1986-01-08     Completed Date:  1986-01-08     Revised Date:  2002-11-01    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  421-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Calibration
Carboxylic Acids / analysis*
Chromatography, Gas
Chromatography, Ion Exchange
Citric Acid Cycle
Species Specificity
Trimethylsilyl Compounds
Triticum / analysis*
Chemical
Reg. No./Substance:
0/Carboxylic Acids; 0/Trimethylsilyl Compounds; 21149-38-2/N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Intrinsic fluorometric determination of the stable state of aggregation in hemoglobins.
Next Document:  A gel transfer tank for immunoblotting and its application for analysis of nuclear protein antigens.