| Determination of organic acid impurities in lactic acid obtained by fermentation of sugarcane juice. | |
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MedLine Citation:
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PMID: 21893318 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Lactic acid produced by fermentation process mostly contains a number of aliphatic carboxylic acids as impurities. In this work, carboxylic acid impurities in lactic acid samples from a number of sources were determined at ppm levels. A simple HPLC method was developed that utilized a new generation polar embedded reverse phase, 20mM phosphate buffer at pH 2.20 (±0.05) and UV detection at 210nm. The method enabled quantitative analysis of the above acids in lactic acid matrix. The experimental conditions for column temperature, mobile phase pH and flow rate were optimized. A detailed validation of the method was performed for linearity, precision, accuracy, selectivity, limit of detection (LOD), limit of quantitation (LOQ), ruggedness and repeatability and reproducibility (R&R). |
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Authors:
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Mohd Shadbar Qureshi; Sunil S Bhongale; Archana K Thorave |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-8-17 |
Journal Detail:
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Title: Journal of chromatography. A Volume: - ISSN: 1873-3778 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-9-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Chemical Engineering and Process Development Division, National Chemical Laboratory, Council of Scientific and Industrial Research (CSIR), Pune 411008, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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