Document Detail

Determination of organ substrate oxidation in vivo by measurement of 13CO2 concentration in blood.
MedLine Citation:
PMID:  9597768     Owner:  NLM     Status:  MEDLINE    
Substrate oxidation by various organs in animals as well as in humans is usually studied by experiments in which radioactively labeled substrates are used and the production of 14CO2 is measured. In vivo, substrate oxidation by an organ has, up to now, not been determined by means of stable isotopes. Problems in the determination of the concentration of 13CO2 in blood may have impeded the use of 13C-labeled substrates. For the determination of 13CO2 concentration in blood a direct method for the determination of total CO2 concentration in blood was combined with the determination of the isotope ratio (13C/12C) of CO2 by isotope ratio mass spectrometry. The intra-assay relative standard deviation of the CO2 concentration (mean: 19.26 mmol l-1; n = 7) was 0.8%. The inter-assay relative standard deviation of the CO2 concentration in solutions of a weighed amount of Na2CO3 determined over a 5 year period was 0.64% (mean: 21.99 mmol l-1; n = 22). The intra-assay relative standard deviation of 13C in CO2 was 0.03% (mean 13C/12C: 0.0111557; n = 5). From the 13CO2 concentration in arterial and venous blood, substrate oxidation by various organs can be calculated. As an illustration, the determination of myocardial glucose oxidation in lambs, both at rest and during exercise, is described.
G C Beaufort-Krol; J Takens; M C Molenkamp; G B Smid; W G Zijlstra; J R Kuipers
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of mass spectrometry : JMS     Volume:  33     ISSN:  1076-5174     ISO Abbreviation:  J Mass Spectrom     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-06-04     Completed Date:  1998-06-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9504818     Medline TA:  J Mass Spectrom     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  328-33     Citation Subset:  IM    
Beatrix Children's Hospital, Division of Pediatric Cardiology, University of Groningen, The Netherlands.
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MeSH Terms
Carbon Dioxide / analysis*,  blood*
Carbon Radioisotopes / diagnostic use
Glucose / metabolism*
Mass Spectrometry / methods*
Myocardium / metabolism*
Physical Exertion / physiology
Substrate Specificity
Reg. No./Substance:
0/Carbon Radioisotopes; 124-38-9/Carbon Dioxide; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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