| Determination of the optimized conditions for coupling oligonucleotides with 16-mercaptohexadecanoic acid chemically adsorbed upon Au. | |
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MedLine Citation:
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PMID: 17970584 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A specific 5'-modified amino group oligonucleotide (Primer 1), 15-mers in length, is selectively coupled with the carboxyl terminated 16-mercaptohexadecanoic acid (MHDA) chemically adsorbed on Au and subsequently hybridized with Antisense Primer. The amide-coupling process is of significance to create an intermediate structure for the purpose of adding Primer 1, while the hybridization reaction is relevant to various diagnostic purposes to determine the presence in nucleic acids for a target sequence. In this work, the coupling setting was particularly emphasized by varying commonly used temperatures and pH values with a definite concentration of coupling agents (i.e., 10 mM). The recombination with analogous hybridization treatment was investigated using high resolution X-ray photoelectron spectroscopy and a 75 degrees grazing angle Fourier transform infrared spectrometer. On the basis of the spectroscopic studies, the optimized conditions for the coupling process that is also correlated with the molecular density of subsequent hybridization process on MHDA/Au have been proposed at 37 degrees C and a pH value of 4.5. Therefore, it is pertinent to intensify the joining of short-chain DNA strands by complementary base pairing in diagnostic applications such as the identification of single nucleotide polymorphisms. |
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Authors:
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Yi-Te Wu; Jiunn-Der Liao; Je-Inn Lin; Cheng-Chan Lu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-31 |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 18 ISSN: 1043-1802 ISO Abbreviation: Bioconjug. Chem. Publication Date: 2007 Nov-Dec |
Date Detail:
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Created Date: 2007-11-21 Completed Date: 2008-01-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1897-904 Citation Subset: IM |
Affiliation:
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Department of Materials Science and Engineering, Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Amides / chemistry Gold / chemistry* Molecular Structure Oligonucleotides / chemistry* Palmitic Acids / chemistry* Spectrophotometry, Infrared |
| Chemical | |
Reg. No./Substance:
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0/16-mercaptohexadecanoic acid; 0/Amides; 0/Oligonucleotides; 0/Palmitic Acids; 7440-57-5/Gold |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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