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Determination of oleanolic and ursolic acid in Chinese herbs using HPLC and γ-CD as mobile phase modifier.
MedLine Citation:
PMID:  21994210     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
To separate and determine oleanolic acid and ursolic acid, a rapid and accurate HPLC using γ-CD as the mobile phase additive was developed. The effect of CD nature and concentration, and the acidity of the mobile phase on the chromatographic behavior of two bioactive triterpenes were systematically studied. Two bioactive triterpenes were completely separated (R = 3.11) on a Kromasil(®) C(18) column (150×4.6 mm id, 5 μm) with the mobile phase consisting of acetonitrile/0.1% phosphoric acid with 2 mM γ-CD as the mobile phase modifier (60:40, v/v). The flow rate was set at 1.0 mL/min and the eluent was detected at 210 nm for two bioactive triterpenes. The linearity of the method was excellent (r=0.9999) over the studied range of 6-300 μg/mL for oleanolic acid, and 12-600 μg/mL for ursolic acid. The LOD and LOQ were 1.5 and 5.0, 1.0 and 3.0 μg/mL for oleanolic acid and ursolic acid, respectively. The optimized method was successfully applied to separate and determine two bioactive triterpenes in five Chinese herbs. It is concluded that this method could be used for rapid and accurate qualitative and quantitative analysis of the two bioactive triterpenes in Chinese herbs.
Authors:
Changhe Wang; Haijing Liu; Binghua Zhang; Huanying Guo
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-12
Journal Detail:
Title:  Journal of separation science     Volume:  -     ISSN:  1615-9314     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Shaanxi Institute for Food and Drug Control, Xi'an, P. R. China.
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