| Determination of nucleic acids using fluorescence probe sensitized by microemulsion. | |
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MedLine Citation:
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PMID: 17225609 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Because the fluorescence of azur A can be quenched by adding nucleic acid, a sensitive fluorometric method for determination of nucleic acids at nanogram levels was established. Using optimal conditions, the calibration curves were linear in the range of 0-6.0 microg/mL for calf thymus deoxyribonucleic acid (ct DNA) and 0-7.0 microg/mL for herring sperm DNA (hs DNA). The limits of determination were 3.5 and 3.8 ng/mL, respectively, which shows the high sensitivity of this method. Triton X-100 microemulsion was applied as a sensitive media to enhance the sensitivity. The binding mode concerning the interactions of azur A with nucleic acids was also studied and the association constant with different binding numbers was obtained. The method has been applied to the determination of nucleic acid in both synthetic and real samples, such as cauliflower and pork liver, with satisfactory results. |
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Authors:
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Qin Wei; Hui Zhang; Bin Du; Caihong Duan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of AOAC International Volume: 89 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2006 Nov-Dec |
Date Detail:
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Created Date: 2007-01-17 Completed Date: 2007-02-09 Revised Date: 2008-03-17 |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 1609-16 Citation Subset: IM |
Affiliation:
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Jinan University, School of Chemistry and Chemical Engineering, Jinan 250022, People's Republic of China. sdjndxwq@263.net |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Animals Azure Stains / chemistry Brassica / chemistry DNA / analysis Emulsions Fluorescent Dyes Hydrogen-Ion Concentration Liver / chemistry Nucleic Acid Denaturation Nucleic Acids / analysis* Octoxynol Reference Standards Solutions Spectrometry, Fluorescence Surface-Active Agents Swine |
| Chemical | |
Reg. No./Substance:
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0/Azure Stains; 0/Emulsions; 0/Fluorescent Dyes; 0/Nucleic Acids; 0/Solutions; 0/Surface-Active Agents; 531-53-3/Azure A; 9002-93-1/Octoxynol; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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