| Determination of nucleic acids based on the quenching effect on resonance light scattering of the Y(III)-1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolone-4'-)hexane-dione system. | |
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MedLine Citation:
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PMID: 16502393 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleic acids can quench resonance light scattering (RLS) intensity of the Y(III)-1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolone-4'-)hexane-dione(BPMPHD) complex in the pH range 5.0-5.8. Under optimal conditions, there are linear relationships between the quenching of RLS and the concentration of nucleic acids in the range 6.3 x 10(-8)-2.1 x 10(-5) g/mL for fish sperm DNA (fsDNA), 1.2 x 10(-8)-5.0 x 10(-5) g/mL for calf thymus DNA (ctDNA) and 6.0 x 10(-8)-2.0 x 10(-5) g/mL for yeast RNA (yRNA). The detection limits (3 s) of fsDNA, ctDNA and yRNA are 0.7 ng/mL, 3.8 ng/mL and 4.2 ng/mL, respectively. |
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Authors:
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Xia Wu; Jing He Yang; Shuna Sun; Changying Guo; Dehuan Ran; Jinhua Zheng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Luminescence : the journal of biological and chemical luminescence Volume: 21 ISSN: 1522-7235 ISO Abbreviation: Luminescence Publication Date: 2006 May-Jun |
Date Detail:
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Created Date: 2006-05-22 Completed Date: 2006-08-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100889025 Medline TA: Luminescence Country: England |
Other Details:
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Languages: eng Pagination: 129-34 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2006 John Wiley & Sons, Ltd. |
Affiliation:
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Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aldehydes
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chemistry* Animals Cattle DNA / analysis, chemistry Fishes Hydrogen-Ion Concentration Male Nucleic Acids / analysis*, chemistry* Pyrazoles / chemistry* RNA / analysis, chemistry Scattering, Radiation* Sensitivity and Specificity Spermatozoa / metabolism Thymus Gland / metabolism Yeasts / metabolism |
| Chemical | |
Reg. No./Substance:
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0/1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolon-4'-)hexanedione; 0/Aldehydes; 0/Nucleic Acids; 0/Pyrazoles; 63231-63-0/RNA; 9007-49-2/DNA; 91080-16-9/calf thymus DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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