Document Detail


Determination of a novel bile acid sequestrant in rodent diet by near-infrared spectroscopy.
MedLine Citation:
PMID:  10698556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DMP 504 is a high molecular weight polymer currently under development by The DuPont Merck Pharmaceutical Company as a novel bile acid sequestrant to lower serum cholesterol. To assess its safety, DMP 504 is incorporated into rodent diet for oral administration to rats and mice. An analytical method was developed to determine the accuracy and homogeneity of the blends. Since a physical separation or extraction of DMP 504 from the diet was not feasible, near-infrared spectroscopy (near-IR) was employed. The near-IR method provides accurate and precise results for blends containing 1.5-8.0% of DMP 504. Comparison of results at the 1.5% level with a cholate binding referee method is also presented. Both methods provided equivalent results for the 1.5% level.
Authors:
J R Scull; K L Moyer; J S Green; R W Woodeshick; M S Alasandro
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  19     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  1999 May 
Date Detail:
Created Date:  2000-03-21     Completed Date:  2000-03-21     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  903-10     Citation Subset:  IM    
Affiliation:
DuPont Merck Pharmaceuticals Company, Analytical R&D, Wilmington, DE 19880, USA. james.r.scull@dupontpharma.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile Acids and Salts / metabolism
Calibration
Diet*
Polymers / analysis*
Quality Control
Quaternary Ammonium Compounds / analysis*
Reproducibility of Results
Rodentia
Spectroscopy, Near-Infrared / methods*
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/DMP-504; 0/Polymers; 0/Quaternary Ammonium Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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