| Determination of neuroepithelial cell fate: induction of the oligodendrocyte lineage by ventral midline cells and sonic hedgehog. | |
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MedLine Citation:
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PMID: 8660874 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Near the floor plate of the embryonic neural tube there is a group of neuroepithelial precursor cells that are specialized for production of the oligodendrocyte lineage. We performed experiments to test whether specification of these neuroepithelial oligodendrocyte precursors, like other ventral neural cell types, depends on signals from the notochord and/or floor plate. We analyzed heterozygous Danforth's short tail (Sd/+) mutant mice, which lack a notochord and floor plate in caudal regions of the neural tube, and found that oligodendrocyte precursors did not appear at the ventricular surface where there was no floor plate. Moreover, oligodendrocytes did not develop in explant cultures of Sd/+ spinal cord in the absence of a floor plate. When a second notochord was grafted into an ectopic position dorsolateral to the endogenous notochord of a chicken embryo, an additional floor plate was induced along with an ectopic focus of oligodendrocyte precursors at the ventricular surface. Oligodendrocytes developed in explants of intermediate neural tube only when they were cocultured with fragments of notochord or in the presence of purified Sonic hedgehog (Shh) protein. Thus, signals from the notochord/floor plate, possibly involving Shh, are necessary and sufficient to induce the development of ventrally derived oligodendroglia. These signals appear to act by specifying the future fate(s) of neuroepithelial cells at the ventricular surface rather than by influencing the proliferation or differentiation of prespecified progenitor cells in the parenchyma of the cord. |
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Authors:
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N P Pringle; W P Yu; S Guthrie; H Roelink; A Lumsden; A C Peterson; W D Richardson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Developmental biology Volume: 177 ISSN: 0012-1606 ISO Abbreviation: Dev. Biol. Publication Date: 1996 Jul |
Date Detail:
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Created Date: 1996-08-27 Completed Date: 1996-08-27 Revised Date: 2009-09-29 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 30-42 Citation Subset: IM |
Affiliation:
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MRC Laboratory for Molecular Cell Biology and Department of Biology, University College London, Gower Street, London, WC1E 6BT, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Lineage / physiology Cell Movement Cells, Cultured Chick Embryo Embryonic Induction / genetics* Epithelial Cells Epithelium / embryology Female Fetal Tissue Transplantation Hedgehog Proteins In Situ Hybridization Mice Mice, Mutant Strains Microscopy, Confocal Nervous System / cytology, embryology* Notochord / embryology*, transplantation Oligodendroglia / cytology, physiology* Pregnancy Proteins / genetics* Spinal Cord / embryology Stem Cells / physiology* Trans-Activators* |
| Grant Support | |
ID/Acronym/Agency:
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N01-HD-2-3144/HD/NICHD NIH HHS; //Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Hedgehog Proteins; 0/Proteins; 0/Trans-Activators |
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